The effect of murine anti-thymocyte globulin on experimental kidney warm ischemia-reperfusion injury in mice

Hye Ryoun Jang, Maria Teresa Gandolfo, Gang Jee Ko, Lorraine Racusen, Hamid Rabb

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Kidney ischemia-reperfusion injury (IRI) is an important contributor to delayed graft function (DGF) and poor outcome of allografts. Small clinical studies suggest a beneficial role for human anti-thymocyte globulin (ATG) in DGF. We investigated the short-term effect of mouse anti-thymocyte globulin (mATG) on kidney warm IRI in mice. We administered either mATG, rabbit immunoglobulin (RIgG), or saline with different dosing schedules in three different IRI models: 30 min bilateral, 60 min bilateral, and 45 min unilateral IRI. mATG effectively depleted circulating T cells but had less effect on kidney-infiltrating T cells. There was no difference in serum creatinine levels between groups in each study. Scoring of renal tubular damage and regenerating tubules revealed no difference between groups. The percentage of CD3+CD4-CD8- double-negative (DN) T cells, which were reported to contribute to the pathogenesis of lupus nephritis, increased and the percentages of regulatory T cells and NK cells decreased in the post-ischemic kidneys of mATG treated mice. mATG did not alter the expression of pro-inflammatory cytokines such as IFN-γ or anti-inflammatory cytokines such as IL-10 in post-ischemic kidneys. mATG treatment, whether initiated before ischemia or immediately after reperfusion, had minimal effects on renal injury following warm IRI in mice.

Original languageEnglish
Pages (from-to)44-54
Number of pages11
JournalTransplant Immunology
Volume22
Issue number1-2
DOIs
Publication statusPublished - 2009 Jan 1
Externally publishedYes

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Keywords

  • Anti-thymocyte globulin
  • Immune mechanisms
  • Ischemia-reperfusion injury

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Transplantation

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