The effect of platelet rich plasma from bone marrow aspirate with added bone morphogenetic protein-2 on the achilles tendon-bone junction in Rabbits

Hak Jun Kim, Hyok Woo Nam, Chang Yong Hur, Misu Park, Hee Seok Yang, Byung Soo Kim, Jung Ho Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: To determine if exogenously injected bone marrow derived platelet-rich plasma (PRP) plus bone morphogenetic protein (BMP)-2 could accelerate the healing of bone-tendon junction injuries and increase the junction holding strength during the early regeneration period. Methods: A direct injury model of the bone-tendon junction was made using an Achilles tendon-calcaneus bone junction in a rabbit. In the PRP/BMP-2/fibrin group, 0.05 mL of bone marrow derived PRP and 100 ng/mL of BMP-2 both incorporated into 0.1 mL of fibrin glue were injected into Achilles tendon-calcaneus bone junctions. The effect of the intervention was tested by comparing the results of an intervention group to a control group. The results of biomechanical testing, and histological and gross analyses were compared between the 2 groups at the following time points after surgery: 2 weeks, 4 weeks, and 8 weeks. Results: Histologic examinations showed that woven bone developed in tendon-bone junctions at 2 weeks after surgery in the PRP/BMP-2/fibrin group. Mechanical test results showed no significant difference between the PRP/BMP-2/fibrin and control groups at 2 and 4 weeks after surgery, but the mean maximal load in the PRP/BMP-2/fibrin group was significantly higher than in the control group (p < 0.05) at 8 weeks after surgery. Conclusions: Bone marrow derived PRP and BMP-2 in fibrin glue accelerated healing in a rabbit model of tendon-bone junction injury.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalClinics in orthopedic surgery
Volume3
Issue number4
DOIs
Publication statusPublished - 2011 Dec

Keywords

  • Achilles tendon
  • Bone marrow derived platelet rich plasma
  • Bone morphogenetic protein
  • Bone-tendon junction

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

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