The effect of shear stress on transfection of primary neurons using microfluidic platform

Hyung Joon Kim, Jae Hwan Byun, Sudong Kim, Qing Ping Hu, Hyun Ryul Ryu, Yekyuong Cho, Sang Jun Sim, Hwa Sung Shin, Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a novel microfluidic device that was used to investigate the effect of shear stress on transfection efficiency of lipoplex to neurons. The device can be used to simultaneously generate regions with multiple shear stress levels using a single device. When exposed to shear stress, post-mitotic primary rat cortical neurons' transfection rate increased by upto 3-fold when compared to static conventional method. Similar effect was observed with mitotic neuronal cell line NlE-115 where upto 45 % transfection efficiency was achieved with the aid of shear stress. We demonstrated the efficiency of the reversibly binding microfluidic device in executing transfection experiments and corroborated the fact that shear stress is a new parameter to improve non-viral transfection to cells.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1913-1915
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

    Fingerprint

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, H. J., Byun, J. H., Kim, S., Hu, Q. P., Ryu, H. R., Cho, Y., Sim, S. J., Shin, H. S., & Jeon, N. L. (2009). The effect of shear stress on transfection of primary neurons using microfluidic platform. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1913-1915). Chemical and Biological Microsystems Society.