The effect of short-term exposure of triamcinolone acetonide on fibroblasts and retinal pigment epithelial cells

Jae Ryung Oh, You Sun Jung, Geun Soo Kim, In Kyung Oh, Bo Kun Rho, Kuhl Huh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pupose: We investigated the effects of short-term exposure to triamcinolone on cultured choroidal fibroblast (CFB) cells and retinal pigment epithelial (RPE) cells. Methods: To evaluate the effect of triamcinolone on cell proliferation, CFB and RPE cells were divided into three groups: a short-term exposure group; a longterm exposure group, and a non-treated control group. Cells in the short-term exposure group were briefly exposed (5, 15 or 30 mins) to triamcinolone (0.01 mg/ml, 1 mg/ml or mitomycin C (0.01 μg/ml, 1 μg/ml). Cells in the longterm exposure group were continuously incubated in culture medium containing the drug until assessment. The control group was cultured without drugs. Cell viability and the number of cells were assessed at day 5 after exposure. To investigate the direct toxicity of triamcinolone on confluent RPE cells, completely confluent cells were exposed to the drugs in the manner as described above. Cell viability was determined on days 0, 3 and 5 after treatment. Results: In the short-term exposure group, 1 mg ml triamcinolone causeda significant reduction in the proliferation of CFB and RPE cells. The proliferation of CFBs decreased even with exposure to 0.01 mg/ml triamcinolone. In the longterm exposure group, triamcinolone and mitomycin C reduced the proliferation of both CFB and RPE cells. Even very short periods of exposure to triamcinolone caused a significant reduction in the viability of completely confluent RPE cells. Conclusion: Even short periods of exposure to triamcinolone inhibited the proliferation of fibroblasts and RPE cells and were significantly toxic to completely confluent RPE cells.

Original languageEnglish
Pages (from-to)786-790
Number of pages5
JournalActa Ophthalmologica Scandinavica
Volume85
Issue number7
DOIs
Publication statusPublished - 2007 Nov 1

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Triamcinolone
Triamcinolone Acetonide
Retinal Pigments
Fibroblasts
Epithelial Cells
Mitomycin
Cell Survival
Pharmaceutical Preparations
Control Groups
Poisons
Culture Media
Cell Count
Cell Proliferation

Keywords

  • Fibroblast
  • Mitomycin C
  • RPE
  • Triamcinolone

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The effect of short-term exposure of triamcinolone acetonide on fibroblasts and retinal pigment epithelial cells. / Oh, Jae Ryung; Jung, You Sun; Kim, Geun Soo; Oh, In Kyung; Rho, Bo Kun; Huh, Kuhl.

In: Acta Ophthalmologica Scandinavica, Vol. 85, No. 7, 01.11.2007, p. 786-790.

Research output: Contribution to journalArticle

Oh, Jae Ryung ; Jung, You Sun ; Kim, Geun Soo ; Oh, In Kyung ; Rho, Bo Kun ; Huh, Kuhl. / The effect of short-term exposure of triamcinolone acetonide on fibroblasts and retinal pigment epithelial cells. In: Acta Ophthalmologica Scandinavica. 2007 ; Vol. 85, No. 7. pp. 786-790.
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AU - Rho, Bo Kun

AU - Huh, Kuhl

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AB - Pupose: We investigated the effects of short-term exposure to triamcinolone on cultured choroidal fibroblast (CFB) cells and retinal pigment epithelial (RPE) cells. Methods: To evaluate the effect of triamcinolone on cell proliferation, CFB and RPE cells were divided into three groups: a short-term exposure group; a longterm exposure group, and a non-treated control group. Cells in the short-term exposure group were briefly exposed (5, 15 or 30 mins) to triamcinolone (0.01 mg/ml, 1 mg/ml or mitomycin C (0.01 μg/ml, 1 μg/ml). Cells in the longterm exposure group were continuously incubated in culture medium containing the drug until assessment. The control group was cultured without drugs. Cell viability and the number of cells were assessed at day 5 after exposure. To investigate the direct toxicity of triamcinolone on confluent RPE cells, completely confluent cells were exposed to the drugs in the manner as described above. Cell viability was determined on days 0, 3 and 5 after treatment. Results: In the short-term exposure group, 1 mg ml triamcinolone causeda significant reduction in the proliferation of CFB and RPE cells. The proliferation of CFBs decreased even with exposure to 0.01 mg/ml triamcinolone. In the longterm exposure group, triamcinolone and mitomycin C reduced the proliferation of both CFB and RPE cells. Even very short periods of exposure to triamcinolone caused a significant reduction in the viability of completely confluent RPE cells. Conclusion: Even short periods of exposure to triamcinolone inhibited the proliferation of fibroblasts and RPE cells and were significantly toxic to completely confluent RPE cells.

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KW - Mitomycin C

KW - RPE

KW - Triamcinolone

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