The effect of the dissolved oxygen (D.O.) concentration on the production of lignolytic enzymes and isozyme distributions by Phanerochaete chrysosporium was studied in the immobilized reactor system. The oxygen levels significantly affected the production of lignin peroxidase (LiP) and manganese peroxidase (MnP), as well as that of H2O2. It is known that a high oxygen level is required to produce these enzymes. In this study, however, D.O. concentrations above a critical D.O. concentration (1.5 fold increase over the saturated concentration with air) inhibited LiP and MnP productions in the experiments when oxygen was continuously supplied. It is thought that a greater H2O2 accumulation seen with higher D.O. concentrations caused adverse effects on the LiP and MnP productions. On the other hand, with lower D.O. concentrations, H2O2 was not produced enough to stimulate either LiP or MnP production. To reduce excess H2O2 accumulation during culturing with a continuous supply of oxygen, a pulsed supply of oxygen was introduced.
|Number of pages||12|
|Journal||ACS Symposium Series|
|Publication status||Published - 2002|
ASJC Scopus subject areas
- Chemical Engineering(all)