The effect of the oxygen scavenging system on the pH of buffered sample solutions: In the context of single-molecule fluorescence measurements

Sung Eun Kim, Il Buem Lee, Seok Cheol Hong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

Original languageEnglish
Pages (from-to)958-962
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume33
Issue number3
DOIs
Publication statusPublished - 2012 Mar 20

Fingerprint

Scavenging
Fluorescence
Oxygen
Molecules
Photobleaching
Fluorescent Dyes
Experiments

Keywords

  • Gloxy
  • I-motif
  • Oxygen scavenging system
  • PH
  • Single-molecule fluorescence

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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abstract = "In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.",
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T1 - The effect of the oxygen scavenging system on the pH of buffered sample solutions

T2 - In the context of single-molecule fluorescence measurements

AU - Kim, Sung Eun

AU - Lee, Il Buem

AU - Hong, Seok Cheol

PY - 2012/3/20

Y1 - 2012/3/20

N2 - In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

AB - In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

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KW - Oxygen scavenging system

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KW - Single-molecule fluorescence

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JF - Bulletin of the Korean Chemical Society

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