The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity

Su Young Lee, Young Pil Yun, Hae Ryong Song, Heung Jae Chun, Dae Hyeok Yang, Kyeongsoon Park, Sung Eun Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.

Original languageEnglish
Pages (from-to)546-554
Number of pages9
JournalCarbohydrate Polymers
Volume98
Issue number1
DOIs
Publication statusPublished - 2013 Oct 14

Fingerprint

Osteoblasts
Titanium
Heparin
Lysine
Substrates
Osteocalcin
Angle measurement
Coulomb interactions
Contact angle
Surface treatment
Calcium
X ray photoelectron spectroscopy
Scanning electron microscopy

Keywords

  • Bone morphogenic protein-2 (BMP-2)
  • Heparin
  • Osteoblast activity
  • Titanium

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity. / Lee, Su Young; Yun, Young Pil; Song, Hae Ryong; Chun, Heung Jae; Yang, Dae Hyeok; Park, Kyeongsoon; Kim, Sung Eun.

In: Carbohydrate Polymers, Vol. 98, No. 1, 14.10.2013, p. 546-554.

Research output: Contribution to journalArticle

Lee, Su Young ; Yun, Young Pil ; Song, Hae Ryong ; Chun, Heung Jae ; Yang, Dae Hyeok ; Park, Kyeongsoon ; Kim, Sung Eun. / The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity. In: Carbohydrate Polymers. 2013 ; Vol. 98, No. 1. pp. 546-554.
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