The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity

Su Young Lee, Young Pil Yun, Hae Ryong Song, Heung Jae Chun, Dae Hyeok Yang, Kyeongsoon Park, Sung Eun Kim

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.

Original languageEnglish
Pages (from-to)546-554
Number of pages9
JournalCarbohydrate Polymers
Volume98
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Bone morphogenic protein-2 (BMP-2)
  • Heparin
  • Osteoblast activity
  • Titanium

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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