The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity

Su Young Lee; Young Pil Yun; Hae Ryong Song; Heung Jae Chun; Dae Hyeok Yang; Kyeongsoon Park; Sung Eun Kim

doi:10.1016/j.carbpol.2013.05.095

The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity

Su Young Lee, Young Pil Yun, Hae Ryong Song, Heung Jae Chun, Dae Hyeok Yang, Kyeongsoon Park, Sung Eun Kim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.

Original language	English
Pages (from-to)	546-554
Number of pages	9
Journal	Carbohydrate Polymers
Volume	98
Issue number	1
DOIs	https://doi.org/10.1016/j.carbpol.2013.05.095
Publication status	Published - 2013

Keywords

Bone morphogenic protein-2 (BMP-2)
Heparin
Osteoblast activity
Titanium

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2013.05.095

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title = "The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity",

abstract = "The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.",

keywords = "Bone morphogenic protein-2 (BMP-2), Heparin, Osteoblast activity, Titanium",

author = "Lee, {Su Young} and Yun, {Young Pil} and Song, {Hae Ryong} and Chun, {Heung Jae} and Yang, {Dae Hyeok} and Kyeongsoon Park and Kim, {Sung Eun}",

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doi = "10.1016/j.carbpol.2013.05.095",

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AU - Yun, Young Pil

AU - Song, Hae Ryong

AU - Chun, Heung Jae

AU - Yang, Dae Hyeok

AU - Park, Kyeongsoon

AU - Kim, Sung Eun

PY - 2013

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N2 - The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.

AB - The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP- 2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity.

KW - Bone morphogenic protein-2 (BMP-2)

KW - Heparin

KW - Osteoblast activity

KW - Titanium

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The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity

Abstract

Keywords

ASJC Scopus subject areas

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