The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO

Jaejoon Jung, Hyoju Seo, Se Hee Lee, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effects of malachite green (MG) on the bacterial community in Antarctic soil were assessed. Culture-independent community analysis using 16S rRNA gene pyrosequencing showed that, in the presence of MG, the relative abundance of Pseudomonas dramatically increased from 2.2 % to 36.6 % (16.6-fold), and Pseudomonas became the predominant genus. The reduction in bacterial biodiversity was demonstrated by diversity indices and rarefaction curves. MG-degrading Pseudomonas sp. MGO was isolated from Antarctic soil. MG tolerance and decolorization activity were confirmed by growth, spectrophotometric, high-performance liquid chromatography, and thin-layer chromatography analyses in high MG concentrations. Our data showed that the decolorization process occurred via biodegradation, while biosorption also occurred after some time during the fed-batch decolorization process. Significant inductions in laccase, nicotinamide adenine dinucleotide-2,6 dichlorophenol indophenol reductase, and MG reductase activities suggested their involvement in the decolorization process. We also showed that the high tolerance of strain MGO to toxic MG might be mediated by upregulation of oxidative stress defense systems such as superoxide dismutase and protease. Collectively, these results demonstrated the response of the Antarctic soil bacterial community to MG and provided insight into the molecular mechanism of MG-tolerant Pseudomonas strains isolated from Antarctic soil.

Original languageEnglish
Pages (from-to)4511-4521
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume97
Issue number10
DOIs
Publication statusPublished - 2013 May 1

Fingerprint

Poisons
Pseudomonas
Soil
Oxidoreductases
2,6-Dichloroindophenol
malachite green
Laccase
Biodiversity
Thin Layer Chromatography
rRNA Genes
NAD
Superoxide Dismutase
Oxidative Stress
Peptide Hydrolases
Up-Regulation
High Pressure Liquid Chromatography
Growth

Keywords

  • Biodegradation
  • Decolorization
  • Microbial community
  • Superoxide dismutase
  • Toxicity

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO. / Jung, Jaejoon; Seo, Hyoju; Lee, Se Hee; Jeon, Che Ok; Park, Woojun.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 10, 01.05.2013, p. 4511-4521.

Research output: Contribution to journalArticle

@article{503417a5fad944f09dc6b158fa08405a,
title = "The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO",
abstract = "The effects of malachite green (MG) on the bacterial community in Antarctic soil were assessed. Culture-independent community analysis using 16S rRNA gene pyrosequencing showed that, in the presence of MG, the relative abundance of Pseudomonas dramatically increased from 2.2 {\%} to 36.6 {\%} (16.6-fold), and Pseudomonas became the predominant genus. The reduction in bacterial biodiversity was demonstrated by diversity indices and rarefaction curves. MG-degrading Pseudomonas sp. MGO was isolated from Antarctic soil. MG tolerance and decolorization activity were confirmed by growth, spectrophotometric, high-performance liquid chromatography, and thin-layer chromatography analyses in high MG concentrations. Our data showed that the decolorization process occurred via biodegradation, while biosorption also occurred after some time during the fed-batch decolorization process. Significant inductions in laccase, nicotinamide adenine dinucleotide-2,6 dichlorophenol indophenol reductase, and MG reductase activities suggested their involvement in the decolorization process. We also showed that the high tolerance of strain MGO to toxic MG might be mediated by upregulation of oxidative stress defense systems such as superoxide dismutase and protease. Collectively, these results demonstrated the response of the Antarctic soil bacterial community to MG and provided insight into the molecular mechanism of MG-tolerant Pseudomonas strains isolated from Antarctic soil.",
keywords = "Biodegradation, Decolorization, Microbial community, Superoxide dismutase, Toxicity",
author = "Jaejoon Jung and Hyoju Seo and Lee, {Se Hee} and Jeon, {Che Ok} and Woojun Park",
year = "2013",
month = "5",
day = "1",
doi = "10.1007/s00253-012-4669-9",
language = "English",
volume = "97",
pages = "4511--4521",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO

AU - Jung, Jaejoon

AU - Seo, Hyoju

AU - Lee, Se Hee

AU - Jeon, Che Ok

AU - Park, Woojun

PY - 2013/5/1

Y1 - 2013/5/1

N2 - The effects of malachite green (MG) on the bacterial community in Antarctic soil were assessed. Culture-independent community analysis using 16S rRNA gene pyrosequencing showed that, in the presence of MG, the relative abundance of Pseudomonas dramatically increased from 2.2 % to 36.6 % (16.6-fold), and Pseudomonas became the predominant genus. The reduction in bacterial biodiversity was demonstrated by diversity indices and rarefaction curves. MG-degrading Pseudomonas sp. MGO was isolated from Antarctic soil. MG tolerance and decolorization activity were confirmed by growth, spectrophotometric, high-performance liquid chromatography, and thin-layer chromatography analyses in high MG concentrations. Our data showed that the decolorization process occurred via biodegradation, while biosorption also occurred after some time during the fed-batch decolorization process. Significant inductions in laccase, nicotinamide adenine dinucleotide-2,6 dichlorophenol indophenol reductase, and MG reductase activities suggested their involvement in the decolorization process. We also showed that the high tolerance of strain MGO to toxic MG might be mediated by upregulation of oxidative stress defense systems such as superoxide dismutase and protease. Collectively, these results demonstrated the response of the Antarctic soil bacterial community to MG and provided insight into the molecular mechanism of MG-tolerant Pseudomonas strains isolated from Antarctic soil.

AB - The effects of malachite green (MG) on the bacterial community in Antarctic soil were assessed. Culture-independent community analysis using 16S rRNA gene pyrosequencing showed that, in the presence of MG, the relative abundance of Pseudomonas dramatically increased from 2.2 % to 36.6 % (16.6-fold), and Pseudomonas became the predominant genus. The reduction in bacterial biodiversity was demonstrated by diversity indices and rarefaction curves. MG-degrading Pseudomonas sp. MGO was isolated from Antarctic soil. MG tolerance and decolorization activity were confirmed by growth, spectrophotometric, high-performance liquid chromatography, and thin-layer chromatography analyses in high MG concentrations. Our data showed that the decolorization process occurred via biodegradation, while biosorption also occurred after some time during the fed-batch decolorization process. Significant inductions in laccase, nicotinamide adenine dinucleotide-2,6 dichlorophenol indophenol reductase, and MG reductase activities suggested their involvement in the decolorization process. We also showed that the high tolerance of strain MGO to toxic MG might be mediated by upregulation of oxidative stress defense systems such as superoxide dismutase and protease. Collectively, these results demonstrated the response of the Antarctic soil bacterial community to MG and provided insight into the molecular mechanism of MG-tolerant Pseudomonas strains isolated from Antarctic soil.

KW - Biodegradation

KW - Decolorization

KW - Microbial community

KW - Superoxide dismutase

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84877767309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877767309&partnerID=8YFLogxK

U2 - 10.1007/s00253-012-4669-9

DO - 10.1007/s00253-012-4669-9

M3 - Article

C2 - 23296502

AN - SCOPUS:84877767309

VL - 97

SP - 4511

EP - 4521

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 10

ER -