Abstract
Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.
| Original language | English |
|---|---|
| Pages (from-to) | 519-527 |
| Number of pages | 9 |
| Journal | Journal of Hand Surgery |
| Volume | 30 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 May 1 |
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Keywords
- Contractile force
- Methylprednisolone
- Motor function
- S-nitroso-N-acetylcysteine (SNAC)
- Sciatic nerve
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Surgery
Cite this
The effects of exogenous nitric oxide donor on motor functional recovery of reperfused peripheral nerve. / Park, Jong Woong; Qi, Wen Ning; Cai, Yongting; Nunley, James A.; Urbaniak, James R.; Chen, Long En.
In: Journal of Hand Surgery, Vol. 30, No. 3, 01.05.2005, p. 519-527.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of exogenous nitric oxide donor on motor functional recovery of reperfused peripheral nerve
AU - Park, Jong Woong
AU - Qi, Wen Ning
AU - Cai, Yongting
AU - Nunley, James A.
AU - Urbaniak, James R.
AU - Chen, Long En
PY - 2005/5/1
Y1 - 2005/5/1
N2 - Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.
AB - Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.
KW - Contractile force
KW - Methylprednisolone
KW - Motor function
KW - S-nitroso-N-acetylcysteine (SNAC)
KW - Sciatic nerve
UR - http://www.scopus.com/inward/record.url?scp=19944393887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944393887&partnerID=8YFLogxK
U2 - 10.1016/j.jhsa.2004.11.019
DO - 10.1016/j.jhsa.2004.11.019
M3 - Article
C2 - 15925162
AN - SCOPUS:19944393887
VL - 30
SP - 519
EP - 527
JO - Journal of Hand Surgery
JF - Journal of Hand Surgery
SN - 0363-5023
IS - 3
ER -