The effects of exogenous nitric oxide donor on motor functional recovery of reperfused peripheral nerve

Jong Woong Park, Wen Ning Qi, Yongting Cai, James A. Nunley, James R. Urbaniak, Long En Chen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.

Original languageEnglish
Pages (from-to)519-527
Number of pages9
JournalJournal of Hand Surgery
Volume30
Issue number3
DOIs
Publication statusPublished - 2005 May 1

Fingerprint

Nitric Oxide Donors
Peripheral Nerves
Methylprednisolone
Muscles
Phosphates
Sciatic Nerve
Reperfusion
Weights and Measures
S-nitroso-N-acetylcysteine
Reperfusion Injury
Nervous System
Walking
Atrophy
Axons
Regeneration
Histology
Nitric Oxide
Ischemia
Steroids
Therapeutics

Keywords

  • Contractile force
  • Methylprednisolone
  • Motor function
  • S-nitroso-N-acetylcysteine (SNAC)
  • Sciatic nerve

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

The effects of exogenous nitric oxide donor on motor functional recovery of reperfused peripheral nerve. / Park, Jong Woong; Qi, Wen Ning; Cai, Yongting; Nunley, James A.; Urbaniak, James R.; Chen, Long En.

In: Journal of Hand Surgery, Vol. 30, No. 3, 01.05.2005, p. 519-527.

Research output: Contribution to journalArticle

Park, Jong Woong ; Qi, Wen Ning ; Cai, Yongting ; Nunley, James A. ; Urbaniak, James R. ; Chen, Long En. / The effects of exogenous nitric oxide donor on motor functional recovery of reperfused peripheral nerve. In: Journal of Hand Surgery. 2005 ; Vol. 30, No. 3. pp. 519-527.
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AU - Qi, Wen Ning

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AU - Nunley, James A.

AU - Urbaniak, James R.

AU - Chen, Long En

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N2 - Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.

AB - Purpose: To investigate the effects of the nitric oxide donor S-nitroso-N-acetylcysteine (SNAC) on motor functional recovery of reperfused rat sciatic nerve. Methods: Seventy-eight rats were divided into groups treated with SNAC (100 nmol/100 g/min), methylprednisolone 30 mg/kg/h for 15 minutes, 45-minute pause, 5.4 mg/kg/h for 1.5h), and phosphate-buffered saline 0.2 mL/100 g/h). A 1-cm segment of sciatic nerve had 2 hours of ischemia and the results were evaluated after various reperfusion periods using a walking track test, muscle contractile testing, muscle weight, and histology. Results: During reperfusion there was a significant overall improvement in sciatic functional index measurement and isometric titanic contractile force for the SNAC-treated group compared with the methylprednisolone- and phosphate-buffered saline- treated groups. The SNAC group had significantly earlier improvement in the sciatic functional index measurement between days 7 and 28. Restoration of the contractile force and muscle weight of the extensor digitorum longus muscle began earlier in the SNAC group - after day 11 - whereas the other 2 groups showed progressive atrophy until day 21, with a significant difference between the SNAC group and the other 2 groups. Histologic examination showed that SNAC-treated rats had less severe degeneration and earlier regeneration of axons than the others. Although methylprednisolone-treated rats showed earlier recovery than phosphate-buffered saline-treated rats in all parameters there were no significant differences between these 2 groups. Conclusions: Supplementation of nitric oxide is effective in promoting motor functional recovery of the reperfused peripheral nerve and has potential to replace or augment steroids as therapeutic agents in treatment of nervous system ischemia/reperfusion injury.

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