The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity

Sung Eun Kim; Young Pil Yun; Kyeongsoon Park; Hak Jun Kim; Deok Won Lee; Jung Wook Kim; Dae Hyeok Yang; Dong Hun Suh

doi:10.1007/s13770-013-1098-5

The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity

Sung Eun Kim, Young Pil Yun, Kyeongsoon Park, Hak Jun Kim, Deok Won Lee, Jung Wook Kim, Dae Hyeok Yang, Dong Hun Suh

Department of Orthopedic Surgery

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.

Original language	English
Pages (from-to)	353-361
Number of pages	9
Journal	Tissue Engineering and Regenerative Medicine
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s13770-013-1098-5
Publication status	Published - 2013 Jan 1

Keywords

alendronate
bone morphogenic protein-2 (BMP-2)
functionalized titanium (Ti)
osteoblast activity

ASJC Scopus subject areas

Medicine (miscellaneous)
Biomedical Engineering

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Kim, S. E., Yun, Y. P., Park, K., Kim, H. J., Lee, D. W., Kim, J. W., Yang, D. H., & Suh, D. H. (2013). The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity. Tissue Engineering and Regenerative Medicine, 10(6), 353-361. https://doi.org/10.1007/s13770-013-1098-5

The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity. / Kim, Sung Eun; Yun, Young Pil; Park, Kyeongsoon; Kim, Hak Jun; Lee, Deok Won; Kim, Jung Wook; Yang, Dae Hyeok; Suh, Dong Hun.

In: Tissue Engineering and Regenerative Medicine, Vol. 10, No. 6, 01.01.2013, p. 353-361.

Research output: Contribution to journal › Article

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abstract = "The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.",

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