Abstract
The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.
| Original language | English |
|---|---|
| Pages (from-to) | 353-361 |
| Number of pages | 9 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume | 10 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
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Keywords
- alendronate
- bone morphogenic protein-2 (BMP-2)
- functionalized titanium (Ti)
- osteoblast activity
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity. / Kim, Sung Eun; Yun, Young Pil; Park, Kyeongsoon; Kim, Hak Jun; Lee, Deok Won; Kim, Jung Wook; Yang, Dae Hyeok; Suh, Dong Hun.
In: Tissue Engineering and Regenerative Medicine, Vol. 10, No. 6, 01.01.2013, p. 353-361.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity
AU - Kim, Sung Eun
AU - Yun, Young Pil
AU - Park, Kyeongsoon
AU - Kim, Hak Jun
AU - Lee, Deok Won
AU - Kim, Jung Wook
AU - Yang, Dae Hyeok
AU - Suh, Dong Hun
PY - 2013/1/1
Y1 - 2013/1/1
N2 - The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.
AB - The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.
KW - alendronate
KW - bone morphogenic protein-2 (BMP-2)
KW - functionalized titanium (Ti)
KW - osteoblast activity
UR - http://www.scopus.com/inward/record.url?scp=84896704048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896704048&partnerID=8YFLogxK
U2 - 10.1007/s13770-013-1098-5
DO - 10.1007/s13770-013-1098-5
M3 - Article
VL - 10
SP - 353
EP - 361
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
SN - 1738-2696
IS - 6
ER -