The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity

Sung Eun Kim; Young Pil Yun; Kyeongsoon Park; Hak Jun Kim; Deok Won Lee; Jung Wook Kim; Dae Hyeok Yang; Dong Hun Suh

doi:10.1007/s13770-013-1098-5

The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity

Sung Eun Kim, Young Pil Yun, Kyeongsoon Park, Hak Jun Kim, Deok Won Lee, Jung Wook Kim, Dae Hyeok Yang, Dong Hun Suh

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.

Original language	English
Pages (from-to)	353-361
Number of pages	9
Journal	Tissue Engineering and Regenerative Medicine
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s13770-013-1098-5
Publication status	Published - 2013 Dec
Externally published	Yes

Keywords

alendronate
bone morphogenic protein-2 (BMP-2)
functionalized titanium (Ti)
osteoblast activity

ASJC Scopus subject areas

Medicine (miscellaneous)
Biomedical Engineering

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10.1007/s13770-013-1098-5

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The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity. / Kim, Sung Eun; Yun, Young Pil; Park, Kyeongsoon; Kim, Hak Jun; Lee, Deok Won; Kim, Jung Wook; Yang, Dae Hyeok; Suh, Dong Hun.

In: Tissue Engineering and Regenerative Medicine, Vol. 10, No. 6, 12.2013, p. 353-361.

Research output: Contribution to journal › Article › peer-review

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abstract = "The aim of this study was to investigate the effect of functionalized titanium (Ti) with alendronate (Aln) and bone morphogenic protein-2 (BMP-2) for enhancement of osteoblast activity in vitro. Aln and/or BMP-2 were sequentially immobilized to the heparinized-Ti (Hep-Ti) surface. The compositions of pristine Ti and Hep-Ti with or without Aln and/or BMP-2 were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Aln and/or BMP-2 onto Hep-Ti surface were released in a sustained manner. Osteoblast activities on all Ti substrates were investigated by cell proliferation assays, alkaline phosphate (ALP) activity, calcium deposition, gene expressions of osteocalcin and osteopontin. Aln/BMP-2/Hep-Ti significantly enhanced ALP activity, calcium mineral deposition, and gene expressions of osteoblast cells when compared with pristine Ti, Aln/Hep-Ti, and BMP-2/Hep-Ti. From these results, functionalized Ti substrates with alendronate and BMP-2 such as Aln/BMP-2/Hep-Ti are a promising material for the enhanced osteoblast activities in orthopedic and dental fields.",

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The effects of functionalized titanium with alendronate and bone morphogenic protein-2 for improving osteoblast activity

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