The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation

Yoon Ji Choi, Se Keun Jeon, Jungjoo Kim, Yongdoo Park, Seung-Zhoo Yoon, Kyung Sun

Research output: Contribution to journalArticle

Abstract

Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.

Original languageEnglish
Pages (from-to)675-681
Number of pages7
JournalJournal of Anesthesia
Volume26
Issue number5
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Intravenous Anesthetics
Fibroblasts
Thiopental
Midazolam
Propofol
Cell Culture Techniques
Cell Proliferation

Keywords

  • Fibroblast
  • Midazolam
  • Propofol
  • Remifentanil
  • Sedation
  • Transplantation

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation. / Choi, Yoon Ji; Jeon, Se Keun; Kim, Jungjoo; Park, Yongdoo; Yoon, Seung-Zhoo; Sun, Kyung.

In: Journal of Anesthesia, Vol. 26, No. 5, 01.10.2012, p. 675-681.

Research output: Contribution to journalArticle

@article{249f0b74bb3b48b8be6b0d7614b6a0b2,
title = "The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation",
abstract = "Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.",
keywords = "Fibroblast, Midazolam, Propofol, Remifentanil, Sedation, Transplantation",
author = "Choi, {Yoon Ji} and Jeon, {Se Keun} and Jungjoo Kim and Yongdoo Park and Seung-Zhoo Yoon and Kyung Sun",
year = "2012",
month = "10",
day = "1",
doi = "10.1007/s00540-012-1427-1",
language = "English",
volume = "26",
pages = "675--681",
journal = "Journal of Anesthesia",
issn = "0913-8668",
publisher = "Springer Japan",
number = "5",

}

TY - JOUR

T1 - The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation

AU - Choi, Yoon Ji

AU - Jeon, Se Keun

AU - Kim, Jungjoo

AU - Park, Yongdoo

AU - Yoon, Seung-Zhoo

AU - Sun, Kyung

PY - 2012/10/1

Y1 - 2012/10/1

N2 - Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.

AB - Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.

KW - Fibroblast

KW - Midazolam

KW - Propofol

KW - Remifentanil

KW - Sedation

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=84878663109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878663109&partnerID=8YFLogxK

U2 - 10.1007/s00540-012-1427-1

DO - 10.1007/s00540-012-1427-1

M3 - Article

VL - 26

SP - 675

EP - 681

JO - Journal of Anesthesia

JF - Journal of Anesthesia

SN - 0913-8668

IS - 5

ER -