Abstract
Objective: This study was designed to examine whether mesenchymal stem cells (MSCs) transduced with Akt enhance cardiac repair after transplantation into the ischemic porcine heart. Methods: MSCs isolated from porcine bone marrow and transduced with myr-Akt were transplanted into porcine hearts after experimental myocardial infarction (MI) using intracoronary injection [Group I, vehicle; Group II, MSCs; Group III, Akt-MSCs]. Myocardial single photon emission tomography (M-SPECT) was performed to assess myocardial function and the infarcted area. Pigs were also sacrificed for immunohistochemical characterization and histologic analysis. In addition, in vitro assays were performed to examine the resistance of Akt-MSCs to H2O2 stimulation. Results: Transplantation of MSCs into the ischemic porcine myocardium (Group II) increased the left ventricular ejection fraction (ΔLV EF; - 6.3 ± 15.1% versus 0.5 ± 6.4%, P < 0.001) and decreased the Δarea of MI (6.8 ± 5.6% versus - 5.0 ± 5.3%, P < 0.001) compared with the vehicle control (Group I). Transplantation of MSCs transduced with myr-Akt (Group III) resulted in further improvement in ΔLV EF (- 6.3 ± 15.1% versus 5.8 ± 11.3%, P < 0.001) and in Δarea of MI (6.8 ± 5.6% versus - 17.0 ± 7.6%, P < 0.001). Akt-MSCs were more resistant to apoptosis, and the levels of extracellular signal-regulated protein kinase (ERK) activation and vascular endothelial growth factor (VEGF) were higher in H2O2-stimulated Akt-MSCs. Conclusion: Cellular transplantation of Akt-MSCs further enhances the repair of injured myocardium compared to MSC transplantation alone by increasing the number of viable MSCs after cellular transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 530-542 |
| Number of pages | 13 |
| Journal | Cardiovascular Research |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Jun 1 |
| Externally published | Yes |
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Keywords
- Akt
- Mesenchymal stem cell
- Myocardial infarction
- Porcine
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model. / Lim, Sang Yeob; Kim, Yong Sook; Ahn, Youngkeun; Jeong, Myung Ho; Hong, Moon Hwa; Joo, Soo Yeon; Nam, Kwang Il; Cho, Jeong Gwan; Kang, Peter M.; Park, Jong Chun.
In: Cardiovascular Research, Vol. 70, No. 3, 01.06.2006, p. 530-542.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model
AU - Lim, Sang Yeob
AU - Kim, Yong Sook
AU - Ahn, Youngkeun
AU - Jeong, Myung Ho
AU - Hong, Moon Hwa
AU - Joo, Soo Yeon
AU - Nam, Kwang Il
AU - Cho, Jeong Gwan
AU - Kang, Peter M.
AU - Park, Jong Chun
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Objective: This study was designed to examine whether mesenchymal stem cells (MSCs) transduced with Akt enhance cardiac repair after transplantation into the ischemic porcine heart. Methods: MSCs isolated from porcine bone marrow and transduced with myr-Akt were transplanted into porcine hearts after experimental myocardial infarction (MI) using intracoronary injection [Group I, vehicle; Group II, MSCs; Group III, Akt-MSCs]. Myocardial single photon emission tomography (M-SPECT) was performed to assess myocardial function and the infarcted area. Pigs were also sacrificed for immunohistochemical characterization and histologic analysis. In addition, in vitro assays were performed to examine the resistance of Akt-MSCs to H2O2 stimulation. Results: Transplantation of MSCs into the ischemic porcine myocardium (Group II) increased the left ventricular ejection fraction (ΔLV EF; - 6.3 ± 15.1% versus 0.5 ± 6.4%, P < 0.001) and decreased the Δarea of MI (6.8 ± 5.6% versus - 5.0 ± 5.3%, P < 0.001) compared with the vehicle control (Group I). Transplantation of MSCs transduced with myr-Akt (Group III) resulted in further improvement in ΔLV EF (- 6.3 ± 15.1% versus 5.8 ± 11.3%, P < 0.001) and in Δarea of MI (6.8 ± 5.6% versus - 17.0 ± 7.6%, P < 0.001). Akt-MSCs were more resistant to apoptosis, and the levels of extracellular signal-regulated protein kinase (ERK) activation and vascular endothelial growth factor (VEGF) were higher in H2O2-stimulated Akt-MSCs. Conclusion: Cellular transplantation of Akt-MSCs further enhances the repair of injured myocardium compared to MSC transplantation alone by increasing the number of viable MSCs after cellular transplantation.
AB - Objective: This study was designed to examine whether mesenchymal stem cells (MSCs) transduced with Akt enhance cardiac repair after transplantation into the ischemic porcine heart. Methods: MSCs isolated from porcine bone marrow and transduced with myr-Akt were transplanted into porcine hearts after experimental myocardial infarction (MI) using intracoronary injection [Group I, vehicle; Group II, MSCs; Group III, Akt-MSCs]. Myocardial single photon emission tomography (M-SPECT) was performed to assess myocardial function and the infarcted area. Pigs were also sacrificed for immunohistochemical characterization and histologic analysis. In addition, in vitro assays were performed to examine the resistance of Akt-MSCs to H2O2 stimulation. Results: Transplantation of MSCs into the ischemic porcine myocardium (Group II) increased the left ventricular ejection fraction (ΔLV EF; - 6.3 ± 15.1% versus 0.5 ± 6.4%, P < 0.001) and decreased the Δarea of MI (6.8 ± 5.6% versus - 5.0 ± 5.3%, P < 0.001) compared with the vehicle control (Group I). Transplantation of MSCs transduced with myr-Akt (Group III) resulted in further improvement in ΔLV EF (- 6.3 ± 15.1% versus 5.8 ± 11.3%, P < 0.001) and in Δarea of MI (6.8 ± 5.6% versus - 17.0 ± 7.6%, P < 0.001). Akt-MSCs were more resistant to apoptosis, and the levels of extracellular signal-regulated protein kinase (ERK) activation and vascular endothelial growth factor (VEGF) were higher in H2O2-stimulated Akt-MSCs. Conclusion: Cellular transplantation of Akt-MSCs further enhances the repair of injured myocardium compared to MSC transplantation alone by increasing the number of viable MSCs after cellular transplantation.
KW - Akt
KW - Mesenchymal stem cell
KW - Myocardial infarction
KW - Porcine
UR - http://www.scopus.com/inward/record.url?scp=33646676295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646676295&partnerID=8YFLogxK
U2 - 10.1016/j.cardiores.2006.02.016
DO - 10.1016/j.cardiores.2006.02.016
M3 - Article
C2 - 16563361
AN - SCOPUS:33646676295
VL - 70
SP - 530
EP - 542
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 3
ER -