The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury

Madhav Bhatia, Ashok K. Saluja, Bernd Hofbauer, Hong Sik Lee, Jean Louis Frossard, Michael L. Steer

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Conclusion. A completely noninvasive animal model of acute pancreatitis- associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury. Background. Significant pulmonary complications have been known to occur in over 50% of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils. Methods. Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum. Results. Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalInternational Journal of Pancreatology
Volume24
Issue number2
Publication statusPublished - 1998 Nov 23
Externally publishedYes

Fingerprint

Lung Injury
Pancreatitis
Neutrophils
Ethionine
Choline
Neutrophil Activation
Diet
Lung
Peroxidase
Animal Models
Hyperamylasemia
Acinar Cells
Capillary Permeability
Serum
Necrosis

Keywords

  • Acute pancreatitis
  • CDE diet
  • Lung injury
  • Myeloperoxidase
  • Neutrophil sequestration

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury. / Bhatia, Madhav; Saluja, Ashok K.; Hofbauer, Bernd; Lee, Hong Sik; Frossard, Jean Louis; Steer, Michael L.

In: International Journal of Pancreatology, Vol. 24, No. 2, 23.11.1998, p. 77-83.

Research output: Contribution to journalArticle

Bhatia, Madhav ; Saluja, Ashok K. ; Hofbauer, Bernd ; Lee, Hong Sik ; Frossard, Jean Louis ; Steer, Michael L. / The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury. In: International Journal of Pancreatology. 1998 ; Vol. 24, No. 2. pp. 77-83.
@article{104c9fe2abaa4869ad42e8f1d5290eeb,
title = "The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury",
abstract = "Conclusion. A completely noninvasive animal model of acute pancreatitis- associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury. Background. Significant pulmonary complications have been known to occur in over 50{\%} of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils. Methods. Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum. Results. Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.",
keywords = "Acute pancreatitis, CDE diet, Lung injury, Myeloperoxidase, Neutrophil sequestration",
author = "Madhav Bhatia and Saluja, {Ashok K.} and Bernd Hofbauer and Lee, {Hong Sik} and Frossard, {Jean Louis} and Steer, {Michael L.}",
year = "1998",
month = "11",
day = "23",
language = "English",
volume = "24",
pages = "77--83",
journal = "Journal of Gastrointestinal Cancer",
issn = "1941-6628",
publisher = "Humana Press",
number = "2",

}

TY - JOUR

T1 - The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury

AU - Bhatia, Madhav

AU - Saluja, Ashok K.

AU - Hofbauer, Bernd

AU - Lee, Hong Sik

AU - Frossard, Jean Louis

AU - Steer, Michael L.

PY - 1998/11/23

Y1 - 1998/11/23

N2 - Conclusion. A completely noninvasive animal model of acute pancreatitis- associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury. Background. Significant pulmonary complications have been known to occur in over 50% of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils. Methods. Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum. Results. Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.

AB - Conclusion. A completely noninvasive animal model of acute pancreatitis- associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury. Background. Significant pulmonary complications have been known to occur in over 50% of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils. Methods. Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum. Results. Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.

KW - Acute pancreatitis

KW - CDE diet

KW - Lung injury

KW - Myeloperoxidase

KW - Neutrophil sequestration

UR - http://www.scopus.com/inward/record.url?scp=0031771392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031771392&partnerID=8YFLogxK

M3 - Article

C2 - 9816540

AN - SCOPUS:0031771392

VL - 24

SP - 77

EP - 83

JO - Journal of Gastrointestinal Cancer

JF - Journal of Gastrointestinal Cancer

SN - 1941-6628

IS - 2

ER -