TY - JOUR
T1 - The effects of oxygen and treatments in hypoxic conditions in sh-sy5y cells
AU - Cho, Young Duck
AU - Choi, Sung Hyuk
AU - Yoon, Young-Hoon
AU - Kim, Jung-Youn
AU - Park, Sung Jun
AU - Lim, Chae Seung
PY - 2018/1/1
Y1 - 2018/1/1
N2 -
Many patients are admitted to the emergency department due to trauma. Patients with massive hemorrhage and respiratory failure can fall into hypovolemic shock. Thereafter, oxygen is an essential part of the treatment of trauma patients, but the mechanisms of its effects in the management of trauma patients remain unknown. Therefore, we conducted an experiment to apply hypoxia, hyperoxia, and other treatment with the goal of decreasing hypoxic neuronal cells damage, as reflected by cell survival, apoptosis, hydrogen peroxide (H
2
O
2
) production, and hypoxia-inducible factor 1a (HIF) expression in SH-SY5Y cells. Under hypoxic insults, cell survival percentages decreased and apoptosis was seen with increased necrotic cell death. High-pressure oxygen (80% O
2
) had no effect compared with normal-pressure oxygen (20% O
2
). After exposure to hypoxia, H2O2 production and levels of HIF significantly increased compared with normoxia. However, when pentoxifylline (PTX), steroid, and hypertonic saline (HTS) were added after exposure to hypoxic conditions, the production of H2O2 and HIF levels significantly decreased in the groups treated with PTX and HTS. That is, the neuroprotective effect of PTX and HTS alleviated the impacts of hypoxic insulted on neuronal cells.
AB -
Many patients are admitted to the emergency department due to trauma. Patients with massive hemorrhage and respiratory failure can fall into hypovolemic shock. Thereafter, oxygen is an essential part of the treatment of trauma patients, but the mechanisms of its effects in the management of trauma patients remain unknown. Therefore, we conducted an experiment to apply hypoxia, hyperoxia, and other treatment with the goal of decreasing hypoxic neuronal cells damage, as reflected by cell survival, apoptosis, hydrogen peroxide (H
2
O
2
) production, and hypoxia-inducible factor 1a (HIF) expression in SH-SY5Y cells. Under hypoxic insults, cell survival percentages decreased and apoptosis was seen with increased necrotic cell death. High-pressure oxygen (80% O
2
) had no effect compared with normal-pressure oxygen (20% O
2
). After exposure to hypoxia, H2O2 production and levels of HIF significantly increased compared with normoxia. However, when pentoxifylline (PTX), steroid, and hypertonic saline (HTS) were added after exposure to hypoxic conditions, the production of H2O2 and HIF levels significantly decreased in the groups treated with PTX and HTS. That is, the neuroprotective effect of PTX and HTS alleviated the impacts of hypoxic insulted on neuronal cells.
KW - Hydrogen Peroxide
KW - Hypertonic Saline
KW - Hypoxia
KW - Hypoxia-Inducible Factor
KW - Pentoxifylline
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UR - http://www.scopus.com/inward/citedby.url?scp=85064093438&partnerID=8YFLogxK
U2 - 10.1097/SHK.0000000000001041
DO - 10.1097/SHK.0000000000001041
M3 - Article
C2 - 29087986
AN - SCOPUS:85064093438
VL - 50
SP - 449
EP - 454
JO - Shock
JF - Shock
SN - 1073-2322
IS - 4
ER -