The Effects of Propofol on Cardiac Function After 4 Hours of Cold Cardioplegia and Reperfusion

Sung Uk Choi, Hye Won Lee, Hae Ja Lim, Suk Min Yoon, Seong Ho Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To examine whether propofol protects against postischemic myocardial dysfunction and apoptosis during reperfusion after prolonged cold ischemia in isolated rat hearts. Design: A prospective, randomized, controlled study. Setting: A university laboratory. Participants: Animals. Interventions: The isolated hearts of 40 Sprague-Dawley male rats were perfused with modified Krebs-Hennseleit solution for 15 minutes for a stabilization period and 15 minutes for a perfusion period and then underwent 4 hours of global cold ischemia followed by 60 minutes of reperfusion. Four groups were studied (n = 10 for each group). Ten hearts served as an untreated control group. Propofol (2 μmol/L) treatment was performed only before ischemia in the PRE group, only during reperfusion in the POST group, and both before and after ischemia in the ALL group. Measurements and Main Results: Infusion of propofol during reperfusion improved recovery of left ventricular-developed pressure (LVDP) from 61.2% ± 8.5% (control) to 86.3% ± 12.1% (POST) and 74.9% ± 13.2% (ALL, both p < 0.05), whereas preischemic infusion of propofol (64.3% ± 9.7%, PRE) did not improve recovery of LVDP. Infusion of propofol during reperfusion significantly reduced the number of apoptotic cells and led to a smaller infarct size than control and PRE groups (p < 0.05, respectively). Conclusions: Propofol infusion during the reperfusion period produced a cardioprotective effect and inhibited apoptosis of cardiomyocytes in the ischemia-reperfusion model, with prolonged cold ischemia, in isolated rat hearts.

Original languageEnglish
Pages (from-to)678-682
Number of pages5
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume21
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Induced Heart Arrest
Propofol
Reperfusion
Cold Ischemia
Ischemia
Ventricular Pressure
Apoptosis
Control Groups
Cardiac Myocytes
Sprague Dawley Rats
Perfusion
Cell Count

Keywords

  • anesthetics
  • apoptosis
  • heart
  • infarct size
  • propofol
  • rat
  • transplantation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

The Effects of Propofol on Cardiac Function After 4 Hours of Cold Cardioplegia and Reperfusion. / Choi, Sung Uk; Lee, Hye Won; Lim, Hae Ja; Yoon, Suk Min; Chang, Seong Ho.

In: Journal of Cardiothoracic and Vascular Anesthesia, Vol. 21, No. 5, 01.10.2007, p. 678-682.

Research output: Contribution to journalArticle

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abstract = "Objective: To examine whether propofol protects against postischemic myocardial dysfunction and apoptosis during reperfusion after prolonged cold ischemia in isolated rat hearts. Design: A prospective, randomized, controlled study. Setting: A university laboratory. Participants: Animals. Interventions: The isolated hearts of 40 Sprague-Dawley male rats were perfused with modified Krebs-Hennseleit solution for 15 minutes for a stabilization period and 15 minutes for a perfusion period and then underwent 4 hours of global cold ischemia followed by 60 minutes of reperfusion. Four groups were studied (n = 10 for each group). Ten hearts served as an untreated control group. Propofol (2 μmol/L) treatment was performed only before ischemia in the PRE group, only during reperfusion in the POST group, and both before and after ischemia in the ALL group. Measurements and Main Results: Infusion of propofol during reperfusion improved recovery of left ventricular-developed pressure (LVDP) from 61.2{\%} ± 8.5{\%} (control) to 86.3{\%} ± 12.1{\%} (POST) and 74.9{\%} ± 13.2{\%} (ALL, both p < 0.05), whereas preischemic infusion of propofol (64.3{\%} ± 9.7{\%}, PRE) did not improve recovery of LVDP. Infusion of propofol during reperfusion significantly reduced the number of apoptotic cells and led to a smaller infarct size than control and PRE groups (p < 0.05, respectively). Conclusions: Propofol infusion during the reperfusion period produced a cardioprotective effect and inhibited apoptosis of cardiomyocytes in the ischemia-reperfusion model, with prolonged cold ischemia, in isolated rat hearts.",
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