The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture

Yong Park, Seung Jin Lee, In Young Choi, Se Ryeon Lee, Hwa Jung Sung, Jong-Hoon Kim, Young Do Yoo, Dongho Geum, Sun Haeng Kim, Byung Soo Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The use of a mouse embryonic fibroblast (MEF) feeder for culture of embryonic stem cells (ESCs) is a widely accepted method, regardless of the ESCs' origin and type. In this study, we performed the undifferentiated propagation of human ES cell lines (hESCs, H1, and HSF6) and mouse ES cell lines (mESCs, D3, and CE3), which were previously maintained on an MEF feeder, using human placenta-derived fibroblast-like cell (HPC) feeders originated from chorionic villi of women who had undergone therapeutic abortion due to known maternal disease that is aggravated by pregnancy. Moreover, we tried to introduce the HPC feeder for the establishment of inducible pluripotent stem cells (iPSCs) from human placental mesenchymal stem cells (MSCs). On the HPC feeder we were able to propagate ESCs and iPSCs colonies as an undifferentiated state up to the 50th passage and 20th passage, respectively. Maintenance of undifferentiated ESCs was identified by the expression of ALP, SSEA-1, SSEA-4, TRA-81, TRA-60, Oct-4, Nanog, or Rex-1. Also, addition of leukemia inhibitory factor was not required for undifferentiated propagation of mESCs on the HPC feeder. The efficiency and expression of three germ layer markers of embryoid bodies (EBs) from ESCs were satisfactory in both the MEF and HPC group. EBs formed from iPSCs were scant, and differentiation to the three germ layers was identifiable by reverst transcription-polymerase chain reactio (RT-PCR) only in the HPC group. In conclusion, the HPC feeder can efficiently support the undifferentiated propagation of hESCs, mESCs, and iPSCs, suggesting that human placenta may be a useful source of universal feeder cells for hESC, mESC, and iPSC culture.

Original languageEnglish
Pages (from-to)315-328
Number of pages14
JournalCellular Reprogramming
Volume12
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Pluripotent Stem Cells
Embryonic Stem Cells
Placenta
Cell Culture Techniques
Fibroblasts
Embryoid Bodies
Feeder Cells
Germ Layers
CD15 Antigens
Therapeutic Abortion
Leukemia Inhibitory Factor
Chorionic Villi
Cell Line
Mesenchymal Stromal Cells
Maintenance
Mothers
Pregnancy
Mouse Embryonic Stem Cells
Human Embryonic Stem Cells

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Cell Biology

Cite this

@article{6701330d15224f8d960f607e4e8f67c3,
title = "The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture",
abstract = "The use of a mouse embryonic fibroblast (MEF) feeder for culture of embryonic stem cells (ESCs) is a widely accepted method, regardless of the ESCs' origin and type. In this study, we performed the undifferentiated propagation of human ES cell lines (hESCs, H1, and HSF6) and mouse ES cell lines (mESCs, D3, and CE3), which were previously maintained on an MEF feeder, using human placenta-derived fibroblast-like cell (HPC) feeders originated from chorionic villi of women who had undergone therapeutic abortion due to known maternal disease that is aggravated by pregnancy. Moreover, we tried to introduce the HPC feeder for the establishment of inducible pluripotent stem cells (iPSCs) from human placental mesenchymal stem cells (MSCs). On the HPC feeder we were able to propagate ESCs and iPSCs colonies as an undifferentiated state up to the 50th passage and 20th passage, respectively. Maintenance of undifferentiated ESCs was identified by the expression of ALP, SSEA-1, SSEA-4, TRA-81, TRA-60, Oct-4, Nanog, or Rex-1. Also, addition of leukemia inhibitory factor was not required for undifferentiated propagation of mESCs on the HPC feeder. The efficiency and expression of three germ layer markers of embryoid bodies (EBs) from ESCs were satisfactory in both the MEF and HPC group. EBs formed from iPSCs were scant, and differentiation to the three germ layers was identifiable by reverst transcription-polymerase chain reactio (RT-PCR) only in the HPC group. In conclusion, the HPC feeder can efficiently support the undifferentiated propagation of hESCs, mESCs, and iPSCs, suggesting that human placenta may be a useful source of universal feeder cells for hESC, mESC, and iPSC culture.",
author = "Yong Park and Lee, {Seung Jin} and Choi, {In Young} and Lee, {Se Ryeon} and Sung, {Hwa Jung} and Jong-Hoon Kim and Yoo, {Young Do} and Dongho Geum and Kim, {Sun Haeng} and Kim, {Byung Soo}",
year = "2010",
month = "6",
day = "1",
doi = "10.1089/cell.2009.0113",
language = "English",
volume = "12",
pages = "315--328",
journal = "Cellular Reprogramming",
issn = "2152-4971",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture

AU - Park, Yong

AU - Lee, Seung Jin

AU - Choi, In Young

AU - Lee, Se Ryeon

AU - Sung, Hwa Jung

AU - Kim, Jong-Hoon

AU - Yoo, Young Do

AU - Geum, Dongho

AU - Kim, Sun Haeng

AU - Kim, Byung Soo

PY - 2010/6/1

Y1 - 2010/6/1

N2 - The use of a mouse embryonic fibroblast (MEF) feeder for culture of embryonic stem cells (ESCs) is a widely accepted method, regardless of the ESCs' origin and type. In this study, we performed the undifferentiated propagation of human ES cell lines (hESCs, H1, and HSF6) and mouse ES cell lines (mESCs, D3, and CE3), which were previously maintained on an MEF feeder, using human placenta-derived fibroblast-like cell (HPC) feeders originated from chorionic villi of women who had undergone therapeutic abortion due to known maternal disease that is aggravated by pregnancy. Moreover, we tried to introduce the HPC feeder for the establishment of inducible pluripotent stem cells (iPSCs) from human placental mesenchymal stem cells (MSCs). On the HPC feeder we were able to propagate ESCs and iPSCs colonies as an undifferentiated state up to the 50th passage and 20th passage, respectively. Maintenance of undifferentiated ESCs was identified by the expression of ALP, SSEA-1, SSEA-4, TRA-81, TRA-60, Oct-4, Nanog, or Rex-1. Also, addition of leukemia inhibitory factor was not required for undifferentiated propagation of mESCs on the HPC feeder. The efficiency and expression of three germ layer markers of embryoid bodies (EBs) from ESCs were satisfactory in both the MEF and HPC group. EBs formed from iPSCs were scant, and differentiation to the three germ layers was identifiable by reverst transcription-polymerase chain reactio (RT-PCR) only in the HPC group. In conclusion, the HPC feeder can efficiently support the undifferentiated propagation of hESCs, mESCs, and iPSCs, suggesting that human placenta may be a useful source of universal feeder cells for hESC, mESC, and iPSC culture.

AB - The use of a mouse embryonic fibroblast (MEF) feeder for culture of embryonic stem cells (ESCs) is a widely accepted method, regardless of the ESCs' origin and type. In this study, we performed the undifferentiated propagation of human ES cell lines (hESCs, H1, and HSF6) and mouse ES cell lines (mESCs, D3, and CE3), which were previously maintained on an MEF feeder, using human placenta-derived fibroblast-like cell (HPC) feeders originated from chorionic villi of women who had undergone therapeutic abortion due to known maternal disease that is aggravated by pregnancy. Moreover, we tried to introduce the HPC feeder for the establishment of inducible pluripotent stem cells (iPSCs) from human placental mesenchymal stem cells (MSCs). On the HPC feeder we were able to propagate ESCs and iPSCs colonies as an undifferentiated state up to the 50th passage and 20th passage, respectively. Maintenance of undifferentiated ESCs was identified by the expression of ALP, SSEA-1, SSEA-4, TRA-81, TRA-60, Oct-4, Nanog, or Rex-1. Also, addition of leukemia inhibitory factor was not required for undifferentiated propagation of mESCs on the HPC feeder. The efficiency and expression of three germ layer markers of embryoid bodies (EBs) from ESCs were satisfactory in both the MEF and HPC group. EBs formed from iPSCs were scant, and differentiation to the three germ layers was identifiable by reverst transcription-polymerase chain reactio (RT-PCR) only in the HPC group. In conclusion, the HPC feeder can efficiently support the undifferentiated propagation of hESCs, mESCs, and iPSCs, suggesting that human placenta may be a useful source of universal feeder cells for hESC, mESC, and iPSC culture.

UR - http://www.scopus.com/inward/record.url?scp=77954918612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954918612&partnerID=8YFLogxK

U2 - 10.1089/cell.2009.0113

DO - 10.1089/cell.2009.0113

M3 - Article

VL - 12

SP - 315

EP - 328

JO - Cellular Reprogramming

JF - Cellular Reprogramming

SN - 2152-4971

IS - 3

ER -