The elastic property of a single DNA molecule cross-linked by cisplatin: A magnetic tweezers study

Jin Sung Park, Byeongha Jeong, Kyoung Jin Lee, Seok Cheol Hong, Ju Yong Hyon, Seokmann Hong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We observed that the elastic property of a DNA molecule was dramatically changed when the molecule reacted with an anticancer drug, cisplatin. It has been known from bulk assays that cisplatin induces the formation of intra- and inter-strand crosslinks, especially at two neighboring purine sites. From micromanipulation of a single DNA molecule with a magnetic tweezers apparatus, we obtained kinetic information on the interaction between a single DNA molecule and cisplatin, and on the conformation and elastic property of a cisplatin-bound DNA molecule: a single DNA molecule under a constant force was gradually shortened as it interacted with cisplatin, and the elasticity of the DNA molecule revealed by the force vs. extension data was dramatically altered, which indicates that DNA crosslinking by cisplatin is responsible for the changes in the conformation and elasticity. We demonstrated that magnetic tweezers are a versatile tool in studying the mechanical properties of an individual DNA molecule, which have a significant impact on the function of DNA and, therefore, on the anti-cancer effect of cisplatin.

Original languageEnglish
Pages (from-to)963-967
Number of pages5
JournalJournal of the Korean Physical Society
Volume49
Issue number3
Publication statusPublished - 2006 Sep 1

Fingerprint

deoxyribonucleic acid
elastic properties
molecules
strands
purines
crosslinking
drugs
cancer
mechanical properties
kinetics
interactions

Keywords

  • Cisplatin
  • DNA conformation
  • Magnetic tweezers
  • Micromanipulation
  • Single molecule biophysics

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

The elastic property of a single DNA molecule cross-linked by cisplatin : A magnetic tweezers study. / Park, Jin Sung; Jeong, Byeongha; Lee, Kyoung Jin; Hong, Seok Cheol; Hyon, Ju Yong; Hong, Seokmann.

In: Journal of the Korean Physical Society, Vol. 49, No. 3, 01.09.2006, p. 963-967.

Research output: Contribution to journalArticle

@article{8cc96a194f574665b48cf6a84a0a293a,
title = "The elastic property of a single DNA molecule cross-linked by cisplatin: A magnetic tweezers study",
abstract = "We observed that the elastic property of a DNA molecule was dramatically changed when the molecule reacted with an anticancer drug, cisplatin. It has been known from bulk assays that cisplatin induces the formation of intra- and inter-strand crosslinks, especially at two neighboring purine sites. From micromanipulation of a single DNA molecule with a magnetic tweezers apparatus, we obtained kinetic information on the interaction between a single DNA molecule and cisplatin, and on the conformation and elastic property of a cisplatin-bound DNA molecule: a single DNA molecule under a constant force was gradually shortened as it interacted with cisplatin, and the elasticity of the DNA molecule revealed by the force vs. extension data was dramatically altered, which indicates that DNA crosslinking by cisplatin is responsible for the changes in the conformation and elasticity. We demonstrated that magnetic tweezers are a versatile tool in studying the mechanical properties of an individual DNA molecule, which have a significant impact on the function of DNA and, therefore, on the anti-cancer effect of cisplatin.",
keywords = "Cisplatin, DNA conformation, Magnetic tweezers, Micromanipulation, Single molecule biophysics",
author = "Park, {Jin Sung} and Byeongha Jeong and Lee, {Kyoung Jin} and Hong, {Seok Cheol} and Hyon, {Ju Yong} and Seokmann Hong",
year = "2006",
month = "9",
day = "1",
language = "English",
volume = "49",
pages = "963--967",
journal = "Journal of the Korean Physical Society",
issn = "0374-4884",
publisher = "Korean Physical Society",
number = "3",

}

TY - JOUR

T1 - The elastic property of a single DNA molecule cross-linked by cisplatin

T2 - A magnetic tweezers study

AU - Park, Jin Sung

AU - Jeong, Byeongha

AU - Lee, Kyoung Jin

AU - Hong, Seok Cheol

AU - Hyon, Ju Yong

AU - Hong, Seokmann

PY - 2006/9/1

Y1 - 2006/9/1

N2 - We observed that the elastic property of a DNA molecule was dramatically changed when the molecule reacted with an anticancer drug, cisplatin. It has been known from bulk assays that cisplatin induces the formation of intra- and inter-strand crosslinks, especially at two neighboring purine sites. From micromanipulation of a single DNA molecule with a magnetic tweezers apparatus, we obtained kinetic information on the interaction between a single DNA molecule and cisplatin, and on the conformation and elastic property of a cisplatin-bound DNA molecule: a single DNA molecule under a constant force was gradually shortened as it interacted with cisplatin, and the elasticity of the DNA molecule revealed by the force vs. extension data was dramatically altered, which indicates that DNA crosslinking by cisplatin is responsible for the changes in the conformation and elasticity. We demonstrated that magnetic tweezers are a versatile tool in studying the mechanical properties of an individual DNA molecule, which have a significant impact on the function of DNA and, therefore, on the anti-cancer effect of cisplatin.

AB - We observed that the elastic property of a DNA molecule was dramatically changed when the molecule reacted with an anticancer drug, cisplatin. It has been known from bulk assays that cisplatin induces the formation of intra- and inter-strand crosslinks, especially at two neighboring purine sites. From micromanipulation of a single DNA molecule with a magnetic tweezers apparatus, we obtained kinetic information on the interaction between a single DNA molecule and cisplatin, and on the conformation and elastic property of a cisplatin-bound DNA molecule: a single DNA molecule under a constant force was gradually shortened as it interacted with cisplatin, and the elasticity of the DNA molecule revealed by the force vs. extension data was dramatically altered, which indicates that DNA crosslinking by cisplatin is responsible for the changes in the conformation and elasticity. We demonstrated that magnetic tweezers are a versatile tool in studying the mechanical properties of an individual DNA molecule, which have a significant impact on the function of DNA and, therefore, on the anti-cancer effect of cisplatin.

KW - Cisplatin

KW - DNA conformation

KW - Magnetic tweezers

KW - Micromanipulation

KW - Single molecule biophysics

UR - http://www.scopus.com/inward/record.url?scp=33749819944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749819944&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749819944

VL - 49

SP - 963

EP - 967

JO - Journal of the Korean Physical Society

JF - Journal of the Korean Physical Society

SN - 0374-4884

IS - 3

ER -