The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis

Do Hwan Lim, Chun Taek Oh, Langho Lee, Jae Sang Hong, Su Hyun Noh, Seungwoo Hwang, Sungchan Kim, Sung Jun Han, Young Sik Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.

Original languageEnglish
Pages (from-to)3079-3085
Number of pages7
JournalFEBS Letters
Volume585
Issue number19
DOIs
Publication statusPublished - 2011 Oct 3

Keywords

  • Dicer-2
  • Drosophila
  • Endogenous siRNA
  • Lifespan
  • Metabolic homeostasis
  • Stress response

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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