The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway

Sara Kim, Geonhee Hwang, Soohwan Kim, Thom Nguyen Thi, Hanim Kim, Jinkil Jeong, Jaewook Kim, Jungmook Kim, Giltsu Choi, Eunkyoo Oh

Research output: Contribution to journalArticle

Abstract

In plants, an elevation in ambient temperature induces adaptive morphological changes including elongated hypocotyls, which is predominantly regulated by a bHLH transcription factor, PIF4. Although PIF4 is expressed in all aerial tissues including the epidermis, mesophyll, and vascular bundle, its tissue-specific functions in thermomorphogenesis are not known. Here, we show that epidermis-specific expression of PIF4 induces constitutive long hypocotyls, while vasculature-specific expression of PIF4 has no effect on hypocotyl growth. RNA-Seq and qRT-PCR analyses reveal that auxin-responsive genes and growth-related genes are highly activated by epidermal, but not by vascular, PIF4. Additionally, inactivation of epidermal PIF4 or auxin signaling, and overexpression of epidermal phyB suppresses thermoresponsive growth, indicating that epidermal PIF4-auxin pathways are essential for the temperature responses. Further, we show that high temperatures increase both epidermal PIF4 transcription and the epidermal PIF4 DNA-binding ability. Taken together, our study demonstrates that the epidermis regulates thermoresponsive growth through the phyB-PIF4-auxin pathway.

Original languageEnglish
Article number1053
JournalNature communications
Volume11
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Kim, S., Hwang, G., Kim, S., Thi, T. N., Kim, H., Jeong, J., Kim, J., Kim, J., Choi, G., & Oh, E. (2020). The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway. Nature communications, 11(1), [1053]. https://doi.org/10.1038/s41467-020-14905-w