The expression of adiponectin receptor is altered in mild and moderate/severe persistent allergic nasal mucosa

Ha Kyun Kim, Tae-Hoon Kim, Ki Hyoung Kim, Jae Woong Hwang, Sang Heon Park, Gun Whee Yum, Jae Yong Lee, Woo Sung Cho, Heung Man Lee, Seung Hoon Lee, Sang Hag Lee

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3 Citations (Scopus)

Abstract

Background: Adiponectin, one of the adipokines, has been implicated in the inflammatory process in patients with allergic rhinitis. The level of adiponectin is affected by immunotherapy. Considering the fact that adiponectin receptors (AdipoRs) mediate intracellular signaling events in response to the binding of adiponectin, the role of AdipoRs in healthy and allergic nasal mucosa should be determined. This study investigates the level of expression and distribution pattern of AdipoR1 and AdipoR2 in healthy, mild, and moderate/severe persistent allergic nasal mucosa to understand the role of adiponectin in allergic rhinitis. Methods: The level of expression and distribution pattern of AdipoR1 and AdipoR2 were evaluated in healthy, mild, and moderate/severe persistent allergic nasal mucosa, using semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot analysis. Results: AdipoR1 was expressed in healthy, mild, and moderate/severe persistent allergic nasal mucosa where it was commonly localized to the vascular endothelium. However, AdipoR2 was not expressed in any samples of nasal mucosa tested in the present study. Semiquantitative RT-PCR and Western blot analysis showed that the level of expression of AdipoR1 mRNA and protein was decreased in mild and moderate/severe persistent allergic nasal mucosa in comparison with healthy nasal mucosa, but not significantly different between mild and moderate/severe persistent allergic nasal mucosa. Conclusion: These results indicate that AdipoR1 may play an important role in the pathogenesis of allergic nasal mucosa, suggesting a role for AdipoR1 in vascular dysfunction in mild and moderate/severe persistent allergic nasal mucosa.

Original languageEnglish
Pages (from-to)318-322
Number of pages5
JournalAmerican Journal of Rhinology and Allergy
Volume25
Issue number5
DOIs
Publication statusPublished - 2011 Sep 1

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Adiponectin Receptors
Nasal Mucosa
Adiponectin
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
Adipokines
Vascular Endothelium
Immunotherapy
Blood Vessels

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

Cite this

The expression of adiponectin receptor is altered in mild and moderate/severe persistent allergic nasal mucosa. / Kim, Ha Kyun; Kim, Tae-Hoon; Kim, Ki Hyoung; Hwang, Jae Woong; Park, Sang Heon; Yum, Gun Whee; Lee, Jae Yong; Cho, Woo Sung; Lee, Heung Man; Lee, Seung Hoon; Lee, Sang Hag.

In: American Journal of Rhinology and Allergy, Vol. 25, No. 5, 01.09.2011, p. 318-322.

Research output: Contribution to journalArticle

Kim, Ha Kyun ; Kim, Tae-Hoon ; Kim, Ki Hyoung ; Hwang, Jae Woong ; Park, Sang Heon ; Yum, Gun Whee ; Lee, Jae Yong ; Cho, Woo Sung ; Lee, Heung Man ; Lee, Seung Hoon ; Lee, Sang Hag. / The expression of adiponectin receptor is altered in mild and moderate/severe persistent allergic nasal mucosa. In: American Journal of Rhinology and Allergy. 2011 ; Vol. 25, No. 5. pp. 318-322.
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AU - Yum, Gun Whee

AU - Lee, Jae Yong

AU - Cho, Woo Sung

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AU - Lee, Seung Hoon

AU - Lee, Sang Hag

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N2 - Background: Adiponectin, one of the adipokines, has been implicated in the inflammatory process in patients with allergic rhinitis. The level of adiponectin is affected by immunotherapy. Considering the fact that adiponectin receptors (AdipoRs) mediate intracellular signaling events in response to the binding of adiponectin, the role of AdipoRs in healthy and allergic nasal mucosa should be determined. This study investigates the level of expression and distribution pattern of AdipoR1 and AdipoR2 in healthy, mild, and moderate/severe persistent allergic nasal mucosa to understand the role of adiponectin in allergic rhinitis. Methods: The level of expression and distribution pattern of AdipoR1 and AdipoR2 were evaluated in healthy, mild, and moderate/severe persistent allergic nasal mucosa, using semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot analysis. Results: AdipoR1 was expressed in healthy, mild, and moderate/severe persistent allergic nasal mucosa where it was commonly localized to the vascular endothelium. However, AdipoR2 was not expressed in any samples of nasal mucosa tested in the present study. Semiquantitative RT-PCR and Western blot analysis showed that the level of expression of AdipoR1 mRNA and protein was decreased in mild and moderate/severe persistent allergic nasal mucosa in comparison with healthy nasal mucosa, but not significantly different between mild and moderate/severe persistent allergic nasal mucosa. Conclusion: These results indicate that AdipoR1 may play an important role in the pathogenesis of allergic nasal mucosa, suggesting a role for AdipoR1 in vascular dysfunction in mild and moderate/severe persistent allergic nasal mucosa.

AB - Background: Adiponectin, one of the adipokines, has been implicated in the inflammatory process in patients with allergic rhinitis. The level of adiponectin is affected by immunotherapy. Considering the fact that adiponectin receptors (AdipoRs) mediate intracellular signaling events in response to the binding of adiponectin, the role of AdipoRs in healthy and allergic nasal mucosa should be determined. This study investigates the level of expression and distribution pattern of AdipoR1 and AdipoR2 in healthy, mild, and moderate/severe persistent allergic nasal mucosa to understand the role of adiponectin in allergic rhinitis. Methods: The level of expression and distribution pattern of AdipoR1 and AdipoR2 were evaluated in healthy, mild, and moderate/severe persistent allergic nasal mucosa, using semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot analysis. Results: AdipoR1 was expressed in healthy, mild, and moderate/severe persistent allergic nasal mucosa where it was commonly localized to the vascular endothelium. However, AdipoR2 was not expressed in any samples of nasal mucosa tested in the present study. Semiquantitative RT-PCR and Western blot analysis showed that the level of expression of AdipoR1 mRNA and protein was decreased in mild and moderate/severe persistent allergic nasal mucosa in comparison with healthy nasal mucosa, but not significantly different between mild and moderate/severe persistent allergic nasal mucosa. Conclusion: These results indicate that AdipoR1 may play an important role in the pathogenesis of allergic nasal mucosa, suggesting a role for AdipoR1 in vascular dysfunction in mild and moderate/severe persistent allergic nasal mucosa.

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