The expression of epidermal growth factor receptor, vascular endothelial growth factor, matrix metalloproteinase-2, and cyclooxygenase-2 in relation to human papilloma viral load and persistence of human papillomavirus after conization with negative margins

S. H. Song, Jae Kwan Lee, Jun Young Hur, I. Kim, H. S. Saw, Y. K. Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The aim of this study was to investigate the correlations between human papillomavirus (HPV) load and vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 (MMP-2), and cyclooxygenase-2 (COX-2), and to identify biomarkers that may predict high-risk HPV clearance or persistence after conization with negative margins. The following samples were analyzed: 77 paraffin-embedded specimens from patients with cervical intraepithelial neoplasia (CIN), including 27 CIN 2 conization specimens and 50 CIN 3 conization specimens. Immunohistochemical analysis was performed with antibodies to VEGF, EGFR, MMP-2, and COX-2. Hybrid capture II testing was used to detect HPV DNA. VEGF expression was significantly associated with HPV load (ρ = 0.27186, P = 0.0191), while COX-2 expression was significantly and inversely associated with HPV load (ρ = -0.34309, P = 0.0028). In univariate analysis, HPV load (P = 0.0112) and VEGF expression (P = 0.0274) were significantly associated with high-risk HPV clearance or persistence after conization with negative margins. In multiple regression analysis, high viral load (relative light unit/positive control > 500) and positive VEGF expression were significantly associated with high-risk HPV persistence after conization with negative margins (odds ratio [OR]: 9.915, CI: 1.891-51.994; OR: 6.661, CI: 1.208-36.722, respectively). In conclusion, VEGF expression is related to HPV load, while COX-2 expression is inversely related to HPV load, and immunohistochemical analysis of VEGF expression and HPV viral load are a significant and an independent prognostic indicator of high-risk HPV persistence after conization with negative margins.

Original languageEnglish
Pages (from-to)2009-2017
Number of pages9
JournalInternational Journal of Gynecological Cancer
Volume16
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

Fingerprint

Conization
Matrix Metalloproteinase 2
Papilloma
Cyclooxygenase 2
Viral Load
Epidermal Growth Factor Receptor
Vascular Endothelial Growth Factor A
Cervical Intraepithelial Neoplasia
Odds Ratio
Paraffin
Biomarkers

Keywords

  • Conization
  • Cyclooxygenase-2 (COX-2)
  • Epidermal growth factor receptor (EGFR)
  • Matrix metalloproteinase-2 (MMP-2)
  • Vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology
  • Cancer Research

Cite this

@article{558d353d8f064e95ab7f4a5916ce8c82,
title = "The expression of epidermal growth factor receptor, vascular endothelial growth factor, matrix metalloproteinase-2, and cyclooxygenase-2 in relation to human papilloma viral load and persistence of human papillomavirus after conization with negative margins",
abstract = "The aim of this study was to investigate the correlations between human papillomavirus (HPV) load and vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 (MMP-2), and cyclooxygenase-2 (COX-2), and to identify biomarkers that may predict high-risk HPV clearance or persistence after conization with negative margins. The following samples were analyzed: 77 paraffin-embedded specimens from patients with cervical intraepithelial neoplasia (CIN), including 27 CIN 2 conization specimens and 50 CIN 3 conization specimens. Immunohistochemical analysis was performed with antibodies to VEGF, EGFR, MMP-2, and COX-2. Hybrid capture II testing was used to detect HPV DNA. VEGF expression was significantly associated with HPV load (ρ = 0.27186, P = 0.0191), while COX-2 expression was significantly and inversely associated with HPV load (ρ = -0.34309, P = 0.0028). In univariate analysis, HPV load (P = 0.0112) and VEGF expression (P = 0.0274) were significantly associated with high-risk HPV clearance or persistence after conization with negative margins. In multiple regression analysis, high viral load (relative light unit/positive control > 500) and positive VEGF expression were significantly associated with high-risk HPV persistence after conization with negative margins (odds ratio [OR]: 9.915, CI: 1.891-51.994; OR: 6.661, CI: 1.208-36.722, respectively). In conclusion, VEGF expression is related to HPV load, while COX-2 expression is inversely related to HPV load, and immunohistochemical analysis of VEGF expression and HPV viral load are a significant and an independent prognostic indicator of high-risk HPV persistence after conization with negative margins.",
keywords = "Conization, Cyclooxygenase-2 (COX-2), Epidermal growth factor receptor (EGFR), Matrix metalloproteinase-2 (MMP-2), Vascular endothelial growth factor (VEGF)",
author = "Song, {S. H.} and Lee, {Jae Kwan} and Hur, {Jun Young} and I. Kim and Saw, {H. S.} and Park, {Y. K.}",
year = "2006",
month = "11",
day = "1",
doi = "10.1111/j.1525-1438.2006.00727.x",
language = "English",
volume = "16",
pages = "2009--2017",
journal = "International journal of gynecological cancer : official journal of the International Gynecological Cancer Society",
issn = "1048-891X",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - The expression of epidermal growth factor receptor, vascular endothelial growth factor, matrix metalloproteinase-2, and cyclooxygenase-2 in relation to human papilloma viral load and persistence of human papillomavirus after conization with negative margins

AU - Song, S. H.

AU - Lee, Jae Kwan

AU - Hur, Jun Young

AU - Kim, I.

AU - Saw, H. S.

AU - Park, Y. K.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - The aim of this study was to investigate the correlations between human papillomavirus (HPV) load and vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 (MMP-2), and cyclooxygenase-2 (COX-2), and to identify biomarkers that may predict high-risk HPV clearance or persistence after conization with negative margins. The following samples were analyzed: 77 paraffin-embedded specimens from patients with cervical intraepithelial neoplasia (CIN), including 27 CIN 2 conization specimens and 50 CIN 3 conization specimens. Immunohistochemical analysis was performed with antibodies to VEGF, EGFR, MMP-2, and COX-2. Hybrid capture II testing was used to detect HPV DNA. VEGF expression was significantly associated with HPV load (ρ = 0.27186, P = 0.0191), while COX-2 expression was significantly and inversely associated with HPV load (ρ = -0.34309, P = 0.0028). In univariate analysis, HPV load (P = 0.0112) and VEGF expression (P = 0.0274) were significantly associated with high-risk HPV clearance or persistence after conization with negative margins. In multiple regression analysis, high viral load (relative light unit/positive control > 500) and positive VEGF expression were significantly associated with high-risk HPV persistence after conization with negative margins (odds ratio [OR]: 9.915, CI: 1.891-51.994; OR: 6.661, CI: 1.208-36.722, respectively). In conclusion, VEGF expression is related to HPV load, while COX-2 expression is inversely related to HPV load, and immunohistochemical analysis of VEGF expression and HPV viral load are a significant and an independent prognostic indicator of high-risk HPV persistence after conization with negative margins.

AB - The aim of this study was to investigate the correlations between human papillomavirus (HPV) load and vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 (MMP-2), and cyclooxygenase-2 (COX-2), and to identify biomarkers that may predict high-risk HPV clearance or persistence after conization with negative margins. The following samples were analyzed: 77 paraffin-embedded specimens from patients with cervical intraepithelial neoplasia (CIN), including 27 CIN 2 conization specimens and 50 CIN 3 conization specimens. Immunohistochemical analysis was performed with antibodies to VEGF, EGFR, MMP-2, and COX-2. Hybrid capture II testing was used to detect HPV DNA. VEGF expression was significantly associated with HPV load (ρ = 0.27186, P = 0.0191), while COX-2 expression was significantly and inversely associated with HPV load (ρ = -0.34309, P = 0.0028). In univariate analysis, HPV load (P = 0.0112) and VEGF expression (P = 0.0274) were significantly associated with high-risk HPV clearance or persistence after conization with negative margins. In multiple regression analysis, high viral load (relative light unit/positive control > 500) and positive VEGF expression were significantly associated with high-risk HPV persistence after conization with negative margins (odds ratio [OR]: 9.915, CI: 1.891-51.994; OR: 6.661, CI: 1.208-36.722, respectively). In conclusion, VEGF expression is related to HPV load, while COX-2 expression is inversely related to HPV load, and immunohistochemical analysis of VEGF expression and HPV viral load are a significant and an independent prognostic indicator of high-risk HPV persistence after conization with negative margins.

KW - Conization

KW - Cyclooxygenase-2 (COX-2)

KW - Epidermal growth factor receptor (EGFR)

KW - Matrix metalloproteinase-2 (MMP-2)

KW - Vascular endothelial growth factor (VEGF)

UR - http://www.scopus.com/inward/record.url?scp=33845533977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845533977&partnerID=8YFLogxK

U2 - 10.1111/j.1525-1438.2006.00727.x

DO - 10.1111/j.1525-1438.2006.00727.x

M3 - Article

VL - 16

SP - 2009

EP - 2017

JO - International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

JF - International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

SN - 1048-891X

IS - 6

ER -