The expression profile and function of Satb2 in zebrafish embryonic development

Hyun Jong Ahn, Yoojin Park, Suhyun Kim, Hae Chul Park, Su Kyoung Seo, Sang Yeob Yeo, Dongho Geum

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The present study shows the expression profile and function of the homeobox gene, satb2 during zebrafish embryonic development. Satb2 was ubiquitously expressed from the 1 cell stage to the 10-somite stage in zebrafish embryos. Satb2 showed stage-specific expression profiles such as in the pronephric duct at 24 hpf, the branchial arches at 36 hpf, and the ganglion cell layer of the retina and fins at 48 hpf. Additionally, satb2 knockdown embryos were arrested at 50-60% epiboly, and transplantation experiments with satb2 knockdown cells showed migration defects. Interestingly, satb2 knockdown cells also exhibited down-regulation of dynamin II and VAMP4, which are involved in exocytosis and endocytosis, respectively. Furthermore, satb2 knockdown cells have a disorganized actin distribution and an underdeveloped external yolk syncytial layer, both of which are involved in epiboly. These results suggest that satb2 has a functional role in epiboly. This role may potentially be the regulation of endo-exocytic vesicle transport-dependent cell migration and/or the regulation of the development of the yolk syncytial layer.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalMolecules and Cells
Volume30
Issue number4
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Zebrafish
Embryonic Development
Cell Movement
Dynamin II
Embryonic Structures
Branchial Region
Transport Vesicles
Somites
Homeobox Genes
Exocytosis
Endocytosis
Ganglia
Retina
Actins
Down-Regulation
Transplantation

Keywords

  • epiboly
  • Satb2
  • yolk syncytial layer
  • zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The expression profile and function of Satb2 in zebrafish embryonic development. / Ahn, Hyun Jong; Park, Yoojin; Kim, Suhyun; Park, Hae Chul; Seo, Su Kyoung; Yeo, Sang Yeob; Geum, Dongho.

In: Molecules and Cells, Vol. 30, No. 4, 01.10.2010, p. 377-382.

Research output: Contribution to journalArticle

Ahn, Hyun Jong ; Park, Yoojin ; Kim, Suhyun ; Park, Hae Chul ; Seo, Su Kyoung ; Yeo, Sang Yeob ; Geum, Dongho. / The expression profile and function of Satb2 in zebrafish embryonic development. In: Molecules and Cells. 2010 ; Vol. 30, No. 4. pp. 377-382.
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