The functional and histopathologic change in the levator palpebrae superioris and Müller muscle after subconjunctival injection of triamcinolone acetonide

Minwook Chang, Youngseok Lee, Se Hyun Baek

Research output: Contribution to journalArticle

Abstract

Aims: The aim of this study was to evaluate the functional and histopathologic changes in the levator palpebrae superioris and Müller muscles after subconjunctival injection of triamcinolone acetonide (TA) in rabbits. Methods: Twenty-four white New Zealand rabbits were divided into 2 groups. In group A, a subconjunctival injection of 0.5 mL TA (40 mg/mL) was administered to the right eye, whereas a normal saline injection of the same volume was administered to the left eye. In group B, the same procedures were done with a 1.0-mL injection of TA or normal saline into each eyelid. Follow-up was done to evaluate the histopathologic changes in the levator and Müller muscles, changes in the mean transectional area of Müller muscle, and changes in upper-lid height (marginal reflex distance 1) at 1, 2, 4, and 6 weeks after injection. Western blot analyses were used to determine the levels of myosin light chain phosphorylation and α-smooth muscle actin, which are related to the contractility of Müller muscle. Results: No specific changes in marginal reflex distance 1 were noted in either group A or B. No significant histopathologic changes were found in the levator muscles. However, significant thinning of Müller muscle were found, and myosin light chain phosphorylation and α-smooth muscle actin levels were decreased. This was consistent with the histologic changes of Müller muscle observed in rabbits that received a TA injection. These changes were reversible and influenced by the volume of the injection. Conclusions: Subconjunctival injection of TA into the upper eyelids appears to be temporally influential on both the functional and histopathologic changes of Müller muscle in rabbits. This may be explained by the effect of improvement in lid retraction regardless of the minimal specific change observed in the levator muscle.

Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalJournal of Craniofacial Surgery
Volume26
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Oculomotor Muscles
Triamcinolone Acetonide
Muscles
Injections
Rabbits
Myosin Light Chains
Eyelids
Smooth Muscle
Reflex
Actins
Phosphorylation

Keywords

  • Levator palpebrae superioris
  • Müller muscle
  • Subconjunctival injection
  • Triamcinolone acetonide (TA)

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Medicine(all)

Cite this

@article{f6ccae748fa14c15b8ad9ba73f52b742,
title = "The functional and histopathologic change in the levator palpebrae superioris and M{\"u}ller muscle after subconjunctival injection of triamcinolone acetonide",
abstract = "Aims: The aim of this study was to evaluate the functional and histopathologic changes in the levator palpebrae superioris and M{\"u}ller muscles after subconjunctival injection of triamcinolone acetonide (TA) in rabbits. Methods: Twenty-four white New Zealand rabbits were divided into 2 groups. In group A, a subconjunctival injection of 0.5 mL TA (40 mg/mL) was administered to the right eye, whereas a normal saline injection of the same volume was administered to the left eye. In group B, the same procedures were done with a 1.0-mL injection of TA or normal saline into each eyelid. Follow-up was done to evaluate the histopathologic changes in the levator and M{\"u}ller muscles, changes in the mean transectional area of M{\"u}ller muscle, and changes in upper-lid height (marginal reflex distance 1) at 1, 2, 4, and 6 weeks after injection. Western blot analyses were used to determine the levels of myosin light chain phosphorylation and α-smooth muscle actin, which are related to the contractility of M{\"u}ller muscle. Results: No specific changes in marginal reflex distance 1 were noted in either group A or B. No significant histopathologic changes were found in the levator muscles. However, significant thinning of M{\"u}ller muscle were found, and myosin light chain phosphorylation and α-smooth muscle actin levels were decreased. This was consistent with the histologic changes of M{\"u}ller muscle observed in rabbits that received a TA injection. These changes were reversible and influenced by the volume of the injection. Conclusions: Subconjunctival injection of TA into the upper eyelids appears to be temporally influential on both the functional and histopathologic changes of M{\"u}ller muscle in rabbits. This may be explained by the effect of improvement in lid retraction regardless of the minimal specific change observed in the levator muscle.",
keywords = "Levator palpebrae superioris, M{\"u}ller muscle, Subconjunctival injection, Triamcinolone acetonide (TA)",
author = "Minwook Chang and Youngseok Lee and Baek, {Se Hyun}",
year = "2015",
month = "1",
day = "1",
doi = "10.1097/SCS.0000000000001174",
language = "English",
volume = "26",
pages = "285--289",
journal = "Journal of Craniofacial Surgery",
issn = "1049-2275",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - The functional and histopathologic change in the levator palpebrae superioris and Müller muscle after subconjunctival injection of triamcinolone acetonide

AU - Chang, Minwook

AU - Lee, Youngseok

AU - Baek, Se Hyun

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Aims: The aim of this study was to evaluate the functional and histopathologic changes in the levator palpebrae superioris and Müller muscles after subconjunctival injection of triamcinolone acetonide (TA) in rabbits. Methods: Twenty-four white New Zealand rabbits were divided into 2 groups. In group A, a subconjunctival injection of 0.5 mL TA (40 mg/mL) was administered to the right eye, whereas a normal saline injection of the same volume was administered to the left eye. In group B, the same procedures were done with a 1.0-mL injection of TA or normal saline into each eyelid. Follow-up was done to evaluate the histopathologic changes in the levator and Müller muscles, changes in the mean transectional area of Müller muscle, and changes in upper-lid height (marginal reflex distance 1) at 1, 2, 4, and 6 weeks after injection. Western blot analyses were used to determine the levels of myosin light chain phosphorylation and α-smooth muscle actin, which are related to the contractility of Müller muscle. Results: No specific changes in marginal reflex distance 1 were noted in either group A or B. No significant histopathologic changes were found in the levator muscles. However, significant thinning of Müller muscle were found, and myosin light chain phosphorylation and α-smooth muscle actin levels were decreased. This was consistent with the histologic changes of Müller muscle observed in rabbits that received a TA injection. These changes were reversible and influenced by the volume of the injection. Conclusions: Subconjunctival injection of TA into the upper eyelids appears to be temporally influential on both the functional and histopathologic changes of Müller muscle in rabbits. This may be explained by the effect of improvement in lid retraction regardless of the minimal specific change observed in the levator muscle.

AB - Aims: The aim of this study was to evaluate the functional and histopathologic changes in the levator palpebrae superioris and Müller muscles after subconjunctival injection of triamcinolone acetonide (TA) in rabbits. Methods: Twenty-four white New Zealand rabbits were divided into 2 groups. In group A, a subconjunctival injection of 0.5 mL TA (40 mg/mL) was administered to the right eye, whereas a normal saline injection of the same volume was administered to the left eye. In group B, the same procedures were done with a 1.0-mL injection of TA or normal saline into each eyelid. Follow-up was done to evaluate the histopathologic changes in the levator and Müller muscles, changes in the mean transectional area of Müller muscle, and changes in upper-lid height (marginal reflex distance 1) at 1, 2, 4, and 6 weeks after injection. Western blot analyses were used to determine the levels of myosin light chain phosphorylation and α-smooth muscle actin, which are related to the contractility of Müller muscle. Results: No specific changes in marginal reflex distance 1 were noted in either group A or B. No significant histopathologic changes were found in the levator muscles. However, significant thinning of Müller muscle were found, and myosin light chain phosphorylation and α-smooth muscle actin levels were decreased. This was consistent with the histologic changes of Müller muscle observed in rabbits that received a TA injection. These changes were reversible and influenced by the volume of the injection. Conclusions: Subconjunctival injection of TA into the upper eyelids appears to be temporally influential on both the functional and histopathologic changes of Müller muscle in rabbits. This may be explained by the effect of improvement in lid retraction regardless of the minimal specific change observed in the levator muscle.

KW - Levator palpebrae superioris

KW - Müller muscle

KW - Subconjunctival injection

KW - Triamcinolone acetonide (TA)

UR - http://www.scopus.com/inward/record.url?scp=84921423451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921423451&partnerID=8YFLogxK

U2 - 10.1097/SCS.0000000000001174

DO - 10.1097/SCS.0000000000001174

M3 - Article

VL - 26

SP - 285

EP - 289

JO - Journal of Craniofacial Surgery

JF - Journal of Craniofacial Surgery

SN - 1049-2275

IS - 1

ER -