The generation of iPS cells using non-viral magnetic nanoparticlebased transfection

Chang Hyun Lee, Jung Hyun Kim, Hyun Joo Lee, Kilsoo Jeon, HyeJin Lim, Hye yeon Choi, Eung Ryoung Lee, Seung Hwa Park, Jae-Yong Park, Sunghoi Hong, Soonhag Kim, Ssang Goo Cho

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine.

Original languageEnglish
Pages (from-to)6683-6691
Number of pages9
JournalBiomaterials
Volume32
Issue number28
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Induced Pluripotent Stem Cells
Stem cells
Transfection
Genes
Nanoparticles
Magnets
DNA
Germ Layers
Polyetherimides
Regenerative Medicine
Teratoma
Viral DNA
Dynamic light scattering
Fibroblasts
Viruses
Polymers
Genome
Transmission electron microscopy
Technology

Keywords

  • Embryonic stem cells
  • Induced pluripotent stem cells
  • Non-viral transfection
  • Super paramagnetic nanoparticles

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Lee, C. H., Kim, J. H., Lee, H. J., Jeon, K., Lim, H., Choi, H. Y., ... Cho, S. G. (2011). The generation of iPS cells using non-viral magnetic nanoparticlebased transfection. Biomaterials, 32(28), 6683-6691. https://doi.org/10.1016/j.biomaterials.2011.05.070

The generation of iPS cells using non-viral magnetic nanoparticlebased transfection. / Lee, Chang Hyun; Kim, Jung Hyun; Lee, Hyun Joo; Jeon, Kilsoo; Lim, HyeJin; Choi, Hye yeon; Lee, Eung Ryoung; Park, Seung Hwa; Park, Jae-Yong; Hong, Sunghoi; Kim, Soonhag; Cho, Ssang Goo.

In: Biomaterials, Vol. 32, No. 28, 01.10.2011, p. 6683-6691.

Research output: Contribution to journalArticle

Lee, CH, Kim, JH, Lee, HJ, Jeon, K, Lim, H, Choi, HY, Lee, ER, Park, SH, Park, J-Y, Hong, S, Kim, S & Cho, SG 2011, 'The generation of iPS cells using non-viral magnetic nanoparticlebased transfection', Biomaterials, vol. 32, no. 28, pp. 6683-6691. https://doi.org/10.1016/j.biomaterials.2011.05.070
Lee, Chang Hyun ; Kim, Jung Hyun ; Lee, Hyun Joo ; Jeon, Kilsoo ; Lim, HyeJin ; Choi, Hye yeon ; Lee, Eung Ryoung ; Park, Seung Hwa ; Park, Jae-Yong ; Hong, Sunghoi ; Kim, Soonhag ; Cho, Ssang Goo. / The generation of iPS cells using non-viral magnetic nanoparticlebased transfection. In: Biomaterials. 2011 ; Vol. 32, No. 28. pp. 6683-6691.
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