The generation of iPS cells using non-viral magnetic nanoparticlebased transfection

Chang Hyun Lee, Jung Hyun Kim, Hyun Joo Lee, Kilsoo Jeon, Hye Jin Lim, Hye yeon Choi, Eung Ryoung Lee, Seung Hwa Park, Jae Yong Park, Sunghoi Hong, Soonhag Kim, Ssang Goo Cho

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71 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine.

Original languageEnglish
Pages (from-to)6683-6691
Number of pages9
JournalBiomaterials
Volume32
Issue number28
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • Embryonic stem cells
  • Induced pluripotent stem cells
  • Non-viral transfection
  • Super paramagnetic nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Lee, C. H., Kim, J. H., Lee, H. J., Jeon, K., Lim, H. J., Choi, H. Y., Lee, E. R., Park, S. H., Park, J. Y., Hong, S., Kim, S., & Cho, S. G. (2011). The generation of iPS cells using non-viral magnetic nanoparticlebased transfection. Biomaterials, 32(28), 6683-6691. https://doi.org/10.1016/j.biomaterials.2011.05.070