Abstract
We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D3, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 10-14 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 235 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Jun 9 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes. / Song, Hae Jun; Rossi, Antonello; Ceci, Roberta; Kim, In Gyu; Anzano, Mario A.; Jang, Shyh Ing; De Laurenzi, Vincenzo; Steinert, Peter M.
In: Biochemical and Biophysical Research Communications, Vol. 235, No. 1, 09.06.1997, p. 10-14.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes
AU - Song, Hae Jun
AU - Rossi, Antonello
AU - Ceci, Roberta
AU - Kim, In Gyu
AU - Anzano, Mario A.
AU - Jang, Shyh Ing
AU - De Laurenzi, Vincenzo
AU - Steinert, Peter M.
PY - 1997/6/9
Y1 - 1997/6/9
N2 - We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D3, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.
AB - We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D3, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.
UR - http://www.scopus.com/inward/record.url?scp=0031560908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031560908&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.6717
DO - 10.1006/bbrc.1997.6717
M3 - Article
C2 - 9196026
AN - SCOPUS:0031560908
VL - 235
SP - 10
EP - 14
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -