Abstract
Perilla frutescens leaves are often used in East Asian gourmet food. In this study, we investigated the hepatoprotective effects of caffeic acid (CA), rosmarinic acid (RA), and their combination. P. frutescens contains 1.32 μg CA/mg dry material (DM) and 26.84 μg RA/mg DM analyzed by HPLC-DAD and HPLC-MS. CA remarkably reduced the oxidative damage than rosmarinic acid in an in vitro study. Oral intubation with CA or RA alone for five days was conducted prior to treatment with a single dose of tert-butyl hydroperoxide (0.5. mmol/kg b.w., i.p.), which led to a significant reduction of indicators of hepatic toxicity, such as aspartate aminotransferase, alanine aminotransferase, oxidized glutathione, lipid peroxidation and enzyme activities related to antioxidant such as catalase, glutathione peroxidase and superoxide dismutase. Interestingly, compared to treatment with CA or RA alone, a combination of both compounds more increased the endogenous antioxidant enzymes and glutathione (GSH) and decreased lipid peroxidation in livers. These results suggest that CA from perilla leaves plays a role in the increased hepatic GSH concentration, and shows an additive hepatic protection with RA against oxidative hepatic damage.
| Original language | English |
|---|---|
| Pages (from-to) | 92-99 |
| Number of pages | 8 |
| Journal | Food and Chemical Toxicology |
| Volume | 55 |
| DOIs | |
| Publication status | Published - 2013 May 1 |
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Keywords
- Additive effect
- Caffeic acid
- Hepatic protection
- Perilla frutescens
- Rosmarinic acid
ASJC Scopus subject areas
- Food Science
- Toxicology
Cite this
The hepatoprotection of caffeic acid and rosmarinic acid, major compounds of Perilla frutescens, against t-BHP-induced oxidative liver damage. / Yang, Sung Yong; Hong, Chung Oui; Lee, Gung Pyo; Kim, Cheong Tae; Lee, Kwang Won.
In: Food and Chemical Toxicology, Vol. 55, 01.05.2013, p. 92-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The hepatoprotection of caffeic acid and rosmarinic acid, major compounds of Perilla frutescens, against t-BHP-induced oxidative liver damage
AU - Yang, Sung Yong
AU - Hong, Chung Oui
AU - Lee, Gung Pyo
AU - Kim, Cheong Tae
AU - Lee, Kwang Won
PY - 2013/5/1
Y1 - 2013/5/1
N2 - Perilla frutescens leaves are often used in East Asian gourmet food. In this study, we investigated the hepatoprotective effects of caffeic acid (CA), rosmarinic acid (RA), and their combination. P. frutescens contains 1.32 μg CA/mg dry material (DM) and 26.84 μg RA/mg DM analyzed by HPLC-DAD and HPLC-MS. CA remarkably reduced the oxidative damage than rosmarinic acid in an in vitro study. Oral intubation with CA or RA alone for five days was conducted prior to treatment with a single dose of tert-butyl hydroperoxide (0.5. mmol/kg b.w., i.p.), which led to a significant reduction of indicators of hepatic toxicity, such as aspartate aminotransferase, alanine aminotransferase, oxidized glutathione, lipid peroxidation and enzyme activities related to antioxidant such as catalase, glutathione peroxidase and superoxide dismutase. Interestingly, compared to treatment with CA or RA alone, a combination of both compounds more increased the endogenous antioxidant enzymes and glutathione (GSH) and decreased lipid peroxidation in livers. These results suggest that CA from perilla leaves plays a role in the increased hepatic GSH concentration, and shows an additive hepatic protection with RA against oxidative hepatic damage.
AB - Perilla frutescens leaves are often used in East Asian gourmet food. In this study, we investigated the hepatoprotective effects of caffeic acid (CA), rosmarinic acid (RA), and their combination. P. frutescens contains 1.32 μg CA/mg dry material (DM) and 26.84 μg RA/mg DM analyzed by HPLC-DAD and HPLC-MS. CA remarkably reduced the oxidative damage than rosmarinic acid in an in vitro study. Oral intubation with CA or RA alone for five days was conducted prior to treatment with a single dose of tert-butyl hydroperoxide (0.5. mmol/kg b.w., i.p.), which led to a significant reduction of indicators of hepatic toxicity, such as aspartate aminotransferase, alanine aminotransferase, oxidized glutathione, lipid peroxidation and enzyme activities related to antioxidant such as catalase, glutathione peroxidase and superoxide dismutase. Interestingly, compared to treatment with CA or RA alone, a combination of both compounds more increased the endogenous antioxidant enzymes and glutathione (GSH) and decreased lipid peroxidation in livers. These results suggest that CA from perilla leaves plays a role in the increased hepatic GSH concentration, and shows an additive hepatic protection with RA against oxidative hepatic damage.
KW - Additive effect
KW - Caffeic acid
KW - Hepatic protection
KW - Perilla frutescens
KW - Rosmarinic acid
UR - http://www.scopus.com/inward/record.url?scp=84873263169&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873263169&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2012.12.042
DO - 10.1016/j.fct.2012.12.042
M3 - Article
C2 - 23306788
AN - SCOPUS:84873263169
VL - 55
SP - 92
EP - 99
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
SN - 0278-6915
ER -