The human myotubularin-related protein suppresses the growth of lung carcinoma cells

Young Do Yoo, Sun Mi Cho, Jun Suk Kim, Yoon Soo Chang, Chul Min Ahn, Hyung Jung Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Herein, we report that the human myotubularin-related protein (KIAA0371) induced growth suppression of lung cancer cells. Colony formation assays demonstrated that the exogenous expression of the human myotubularin-related gene induced a significant growth inhibition of H1299 cells in which the expression of the human myotubularin-related gene was undetectable. Colony formation of these cells was reduced by 39% after transfection with this gene, as compared with the control vector. Cells stably transfected with this gene showed a reduction in growth of 48% compared with those cells transfected with the empty vector. We also showed that this negative regulation of the myotubularin-related protein is correlated with cell-cycle arrest at G1. In addition, we found that the cyclin-dependent kinase inhibitor, p27, is induced by increased expression of the human myotubularin-related gene. These results suggest that the human myotubularin-related protein can negatively regulate the growth of lung cancer cells.

Original languageEnglish
Pages (from-to)667-671
Number of pages5
JournalOncology Reports
Volume12
Issue number3
Publication statusPublished - 2004 Sep 1

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Carcinoma
Lung
Growth
Proteins
Genes
Lung Neoplasms
Cyclin-Dependent Kinase Inhibitor p27
G1 Phase Cell Cycle Checkpoints
Transfection
myotubularin

Keywords

  • Cell-cycle arrest
  • Growth inhibition
  • Human myotubularin-related protein
  • p27

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yoo, Y. D., Cho, S. M., Kim, J. S., Chang, Y. S., Ahn, C. M., & Kim, H. J. (2004). The human myotubularin-related protein suppresses the growth of lung carcinoma cells. Oncology Reports, 12(3), 667-671.

The human myotubularin-related protein suppresses the growth of lung carcinoma cells. / Yoo, Young Do; Cho, Sun Mi; Kim, Jun Suk; Chang, Yoon Soo; Ahn, Chul Min; Kim, Hyung Jung.

In: Oncology Reports, Vol. 12, No. 3, 01.09.2004, p. 667-671.

Research output: Contribution to journalArticle

Yoo, YD, Cho, SM, Kim, JS, Chang, YS, Ahn, CM & Kim, HJ 2004, 'The human myotubularin-related protein suppresses the growth of lung carcinoma cells', Oncology Reports, vol. 12, no. 3, pp. 667-671.
Yoo YD, Cho SM, Kim JS, Chang YS, Ahn CM, Kim HJ. The human myotubularin-related protein suppresses the growth of lung carcinoma cells. Oncology Reports. 2004 Sep 1;12(3):667-671.
Yoo, Young Do ; Cho, Sun Mi ; Kim, Jun Suk ; Chang, Yoon Soo ; Ahn, Chul Min ; Kim, Hyung Jung. / The human myotubularin-related protein suppresses the growth of lung carcinoma cells. In: Oncology Reports. 2004 ; Vol. 12, No. 3. pp. 667-671.
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