The hydrolysate of barley straw containing inhibitors can be used to produce cephalosporin C by solvent extraction using ethyl acetate

Sung Bong Kim, Hah Young Yoo, Jun Seok Kim, Seung Wook Kim

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6 Citations (Scopus)


When the solvent extraction of the hydrolysate from barley straw was performed using ethyl acetate (EA), the logarithm of the partition coefficient (log P) of the phenols and furans for EA was found to be more than 1.00, which means that more than 90% of the inhibitors were removed from the hydrolysate layer. Cephalosporin C (CPC) was produced from the hydrolysate of dilute acid pretreatment (DAP) by Acremonium chrysogenum M35. A. chrysogenum M35 was cultured using the hydrolysate and the amount of CPC produced was found to be 10.35 g/L at 144 h. Also, the dry cell weight was about 101.5 g/L at 120 h. The utilization of the hydrolysate for CPC production was effective and the solvent extraction method for the removal of inhibitory substances could contribute to the biorefinery process.

Original languageEnglish
Pages (from-to)2203-2206
Number of pages4
JournalProcess Biochemistry
Issue number12
Publication statusPublished - 2014 Jan 1



  • Dilute acid pretreatment
  • Inhibitory substances
  • Partition coefficient
  • Solvent extraction
  • Xylose fermentation

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

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