The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells

Ahmed Abdal Dayem, Jihye Won, Hui Gwan Goo, Gwang Mo Yang, Dong Sik Seo, Byeong Min Jeon, Hye Yeon Choi, Sang Eun Park, Kyung Min Lim, Seon Ho Jang, Soo Bin Lee, Sang Baek Choi, Kyeongseok Kim, Geun Ho Kang, Gyu Bum Yeon, Dae Sung Kim, Ssang Goo Cho

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Pluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be utilized in future clinical applications.

Original languageEnglish
Article number101700
JournalStem Cell Research
Volume43
DOIs
Publication statusPublished - 2020 Mar

Keywords

  • Adhesion
  • ECM motif
  • Human induced pluripotent stem cell
  • Niche
  • Pluripotency
  • Proliferation
  • Stem cell

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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