The improvement of cephalosporin C production by fed-batch culture of Cephalosporium acremonium M25 using rice oil

Jin Hee Kim, Jung Soo Lim, Seung Wook Kim

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16 Citations (Scopus)


The objective of this study is to improve cephalosporin C (CPC) production by optimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and 6.68%, respectively. CPC production was improved 50% by feeding of 5% rice oil at day 3rd and 5th day during the shake flask culture of C. acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bioreactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about 132% compared to the result with batch flask culture.

Original languageEnglish
Pages (from-to)459-464
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - 2004 Jan 1



  • Cephalosporin C
  • Cephalosporium acremonium
  • Optimization
  • Rice oil
  • Statistical method

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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