The incorporation of GALA peptide into a protein cage for an acid-inducible molecular switch

Seung Hye Choi, Kuiwon Choi, Ick Chan Kwon, Hyung Jun Ahn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Caged proteins have been utilized as a biological container in a wide range of applications from material science to biomedicine, and GALA peptide has been known to undergo coil-to-helix transition upon the increased acidity. In this study, GALA synthetic peptide is incorporated to cage protein by genetic modification. Our engineered caged scaffold retains intact at the physiological pH but dissociate completely at pH 6.0, and the dissociated subunits are re-assembled simply by neutralization to biological pH. This acid-induced dissociation has the potential as molecular switch in vivo as well as in vitro so that the acid-sensitive caged proteins are applicable to drug delivery system for acidic target sites such as tumor. Since our design depends on the conformational transition of GALA peptide, not on removal of characteristic interface observed only in viral capsid-like protein, non-viral caged proteins can also be engineered to have molecular switching function. Therefore, this design for acid-sensitive scaffold would broaden the width of applications in nanotechnology including biomimetic material synthesis and biomedicine.

Original languageEnglish
Pages (from-to)5191-5198
Number of pages8
JournalBiomaterials
Volume31
Issue number19
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Cage protein
  • Disassembly
  • GALA peptide
  • Molecular switch
  • Self-assembly

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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