The influence of glycosylation on secretion, stability, and immunogenicity of recombinant HBV pre-S antigen synthesized in Saccharomyces cerevisiae

Jeewon Lee, Jin Seung Park, Je Young Moon, Ki Yong Kim, Hong Mo Moon

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38 Citations (Scopus)

Abstract

Three types of recombinant pre-S antigens (i.e., pre-S1S2) of hepatitis B virus (HBV) were synthesized in Saccharomyces cerevisiae and secreted into extracellular medium: wild type (pre-S1S2) and two mutant antigens, pre-S1°S2 (Asn15Gln) and pre-S1°S2° (Asn15Gln and Asn123Gln). An N-terminus sequence (Ser5-Ala28) of human interleukin 1β (hIL-1β) was used as synthetic prosequence of recombinant HBV surface antigen (pre-S), secreted from S. cerevisiae. The expression cassette comprised the signal peptide of the killer toxin of Kluyveromyces lactis, the synthetic prosequence above, KEX2 dibasic endopeptidase cleavage site (-Lys-Arg-), and the surface antigen. The recombinant pre-S1S2 and pre-S1°S2 were secreted in the hyper-mannosylated form, while the recombinant pre-S1°S2° was produced without N-glycosylation. It has been demonstrated that the two particular N-linked glycans at Asn15 and Asn123 interfered with the B-cell response to the HBV-derived pre-S1S2, resulting in low titers of pre-S1S2-neutralizing antibodies. This problem was overcome by eliminating both of the N-glycosylation signals. Despite enhanced immunogenicity, the recombinant pre-S1°S2° showed two major problems: (1) inefficient Kex2 cleavage process in the secretory pathway and (2) the severe proteolytic degradation by yeast proteases. The efficiency of Kex2 cleavage increased dramatically by removing N-glycosylation signal in the synthetic prosequence, but the proteolysis of pre-S1°S2° was somewhat inevitable. Further systematic approaches including modulation of degree of N-glycosylation or relocation of N-glycosylation sites in the recombinant pre-S1S2 may make it possible to achieve both enhanced immunogenicity and resistance towards proteolytic degradation of the secreted pre-S antigen.

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number2
DOIs
Publication statusPublished - 2003 Apr 4

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Keywords

  • HBV pre-S
  • Immunogenicity
  • N-glycosylation
  • Proteolysis
  • Secretion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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