The influence of N-glycosylation and C-terminal sequence on secretion of HBV large surface antigen from S. cerevisiae

Jin Seung Park, Hyuk Seong Seo, Jung Sun Yum, Hong Mo Moon, Jeewon Lee

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5 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, we synthesized and secreted L-HBVsAg (named as pre-S(Met1 to Asn174)::S(Met175 to Ile400)) and three mutants, i.e., pre-S ○○::S (Asn15Gln and Asn123Gln), pre-S ○○:: S (Asn15Gln, Asn123Gln, and Asn320Gln), and pre-S ○○::S ○○ (Asn15Gln, Asn123Gln, Asn233Gln, and Asn320Gln). All of the secreted pre-S::S was N-glycosylated, i.e., hyper-mannosylated. In the secretion of pre-S ○○::S and pre-S ○○::S , besides the hyper-mannosylated form, another immunoreactive protein with much lower molecular mass was observed, which seems to be unglycosylated form of pre-S ○○::S and pre-S ○○::S . Only a part of the secreted pre-S ○○::S or pre-S ○○::S molecules was N-glycosylated, and the site for the partial N-glycosylation seems to be Asn233 in S-antigen region. Compared to the N-glycosylated pre-S ○○::S and pre-S ○○::S , pre-S ○○::S ○○ (non-N-glycosylated mutant) was secreted with lower secretion efficiency but showed apparent immunoreactivity to anti-S antigen monoclonal Ab. Interestingly, unlike pre-S ○○::S ○○ with authentic C-terminus, the recombinant pre-S ○○::S ○○ with C-terminal myc or poly-histidine tag (pre-S ○○::S ○○::tag) was almost all aggregated into insoluble proteins in the intracellular region. Conclusively, the C-terminal sequence and glycosylation in S-antigen region seem to be of crucial importance in determining the secretion efficiency of L-HBVsAg in S. cerevisiae.

Original languageEnglish
Pages (from-to)250-255
Number of pages6
JournalBiotechnology and Bioengineering
Volume92
Issue number2
DOIs
Publication statusPublished - 2005 Oct 20

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Keywords

  • C-terminus of S antigen
  • L-HBVsAg
  • N-glycosylation
  • Secretion efficiency

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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