Abstract
Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p<0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.
| Original language | English |
|---|---|
| Pages (from-to) | 171-177 |
| Number of pages | 7 |
| Journal | Biomolecules and Therapeutics |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Jun 9 |
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Keywords
- Baicalin
- CART
- Food intake
- POMC
- Scutellaria baicalensis
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Drug Discovery
- Pharmacology
Cite this
The inhibitory effect of baicalin on the short-term food intake in C57BL/6J mice. / Kim, Eun Ho; Son, Rak Ho; Myoung, Hyeon Jong; Mar, Woongchon; Kim, Won-Ki; Nam, Kung Woo.
In: Biomolecules and Therapeutics, Vol. 18, No. 2, 09.06.2010, p. 171-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The inhibitory effect of baicalin on the short-term food intake in C57BL/6J mice
AU - Kim, Eun Ho
AU - Son, Rak Ho
AU - Myoung, Hyeon Jong
AU - Mar, Woongchon
AU - Kim, Won-Ki
AU - Nam, Kung Woo
PY - 2010/6/9
Y1 - 2010/6/9
N2 - Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p<0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.
AB - Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p<0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.
KW - Baicalin
KW - CART
KW - Food intake
KW - POMC
KW - Scutellaria baicalensis
UR - http://www.scopus.com/inward/record.url?scp=77953103202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953103202&partnerID=8YFLogxK
U2 - 10.4062/biomolther.2010.18.2.171
DO - 10.4062/biomolther.2010.18.2.171
M3 - Article
VL - 18
SP - 171
EP - 177
JO - Biomolecules and Therapeutics
JF - Biomolecules and Therapeutics
SN - 1976-9148
IS - 2
ER -