The inhibitory effect of baicalin on the short-term food intake in C57BL/6J mice

Eun Ho Kim, Rak Ho Son, Hyeon Jong Myoung, Woongchon Mar, Won-Ki Kim, Kung Woo Nam

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p<0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalBiomolecules and Therapeutics
Volume18
Issue number2
DOIs
Publication statusPublished - 2010 Jun 9

Fingerprint

Pro-Opiomelanocortin
Inbred C57BL Mouse
Amphetamine
Cocaine
Eating
Arcuate Nucleus of Hypothalamus
Appetite
Neuropeptides
Luciferases
Hypothalamus
baicalin
Plasmids
Up-Regulation
Down-Regulation

Keywords

  • Baicalin
  • CART
  • Food intake
  • POMC
  • Scutellaria baicalensis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

Cite this

The inhibitory effect of baicalin on the short-term food intake in C57BL/6J mice. / Kim, Eun Ho; Son, Rak Ho; Myoung, Hyeon Jong; Mar, Woongchon; Kim, Won-Ki; Nam, Kung Woo.

In: Biomolecules and Therapeutics, Vol. 18, No. 2, 09.06.2010, p. 171-177.

Research output: Contribution to journalArticle

Kim, Eun Ho ; Son, Rak Ho ; Myoung, Hyeon Jong ; Mar, Woongchon ; Kim, Won-Ki ; Nam, Kung Woo. / The inhibitory effect of baicalin on the short-term food intake in C57BL/6J mice. In: Biomolecules and Therapeutics. 2010 ; Vol. 18, No. 2. pp. 171-177.
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