The inhibitory effect of opioid on the hyperpolarization-activated cation currents in rat substantia gelatinosa neurons

Geun Hee Seol, J. Kim, S. H. Cho, Won-Ki Kim, J. W. Kim, S. J. Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The action of opioid on the hyperpolarization-activated cation current (Ih) in substantia gelatinosa neurons were investigated by using whole-cell voltage-clamp recording in rat spinal brain slices. Hyperpolarizing voltage steps revealed slowly activating currents in a subgroup of neurons. The half-maximal activation and the reversal potential of the current were compatible to neuronal Ih. DAMGO (1 μM), a selective-opioid agonist, reduced the amplitude of Ih reversibly. This reduction was dose-dependent and was blocked by CTOP (2 μM), a selective μ-opioid antagonist. DAMGO shifted the voltage dependence of activation to more hyperpolarized potential. Cesium (1 mM) or ZD 7288 (100 μM) blocked Ih and the currents inhibited by cesium, ZD 7288 and DAMGO shared a similar time and voltage dependence. These results suggest that activation of μ-opioid receptor by DAMGO can inhibit Ih in a subgroup of rat substantia gelatinosa neurons.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume5
Issue number5
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Fingerprint

Substantia Gelatinosa
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Opioid Analgesics
Cations
Neurons
Cesium
Narcotic Antagonists
Opioid Receptors
Brain

Keywords

  • DAMGO
  • Hyperpolarization-activated cation current (I)
  • Opioid
  • Pain
  • Spinal Cord
  • Substantia gelatinosa

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

The inhibitory effect of opioid on the hyperpolarization-activated cation currents in rat substantia gelatinosa neurons. / Seol, Geun Hee; Kim, J.; Cho, S. H.; Kim, Won-Ki; Kim, J. W.; Kim, S. J.

In: Korean Journal of Physiology and Pharmacology, Vol. 5, No. 5, 01.01.2001, p. 373-380.

Research output: Contribution to journalArticle

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