The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone

Sung Woo Jung, Mitsushige Sugimoto, Seiji Shiota, David Y. Graham, Yoshio Yamaoka

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The duodenal ulcer promoting (dupA) gene, located in the plasticity region of Helicobacter pylori, is associated with duodenal ulcer development. dupA was predicted to form a type IV secretory system (T4SS) with vir genes around dupA (dupA cluster). We investigated the prevalence of dupA and dupA clusters and clarified associations between the dupA cluster status and clinical outcomes in the U.S. population. In all, 245 H. pylori strains were examined using PCR to evaluate the status of dupA and the adjacent vir genes predicted to form T4SS, in addition to the status of cag pathogenicity island (PAI). The associations between dupA cluster status and interleukin-8 (IL-8) and IL-12 production were also examined. The presence of dupA and all adjacent vir genes were defined as a complete dupA cluster. Many variations related to the status of dupA and dupA cluster genes were identified. Concurrent H. pylori infection and the presence of a complete dupA cluster increases duodenal ulcer risk compared to H. pylori infection with incomplete dupA cluster or without the dupA gene independent on the cag PAI status (adjusted odds ratio, 2.13; 95% confidence interval, 1.13 to 4.03). Gastric mucosal IL-8 levels were also significantly higher in the complete dupA cluster group than in other groups (P=0.01). In conclusion, although the causal relationship between the dupA cluster and duodenal ulcer development is not proved, the presence of a complete dupA cluster but not dupA alone, is associated with duodenal ulcer development.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalInfection and Immunity
Volume80
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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Duodenal Ulcer
Helicobacter pylori
Virulence
Genomic Islands
Genes
Helicobacter Infections
Interleukin-8
Interleukin-12
Multigene Family
Stomach
Odds Ratio
Confidence Intervals
Polymerase Chain Reaction
Population

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone. / Jung, Sung Woo; Sugimoto, Mitsushige; Shiota, Seiji; Graham, David Y.; Yamaoka, Yoshio.

In: Infection and Immunity, Vol. 80, No. 1, 01.01.2012, p. 381-387.

Research output: Contribution to journalArticle

Jung, Sung Woo ; Sugimoto, Mitsushige ; Shiota, Seiji ; Graham, David Y. ; Yamaoka, Yoshio. / The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone. In: Infection and Immunity. 2012 ; Vol. 80, No. 1. pp. 381-387.
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