The interaction between E-tropomodulin and thymosin β-10 rescues tumor cells from thymosin β-10 mediated apoptosis by restoring actin architecture

Seung Bae Rho, Taehoon Chun, Seung Hoon Lee, Kyoungsook Park, Je Ho Lee

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Thymosin β-10 (TB10) is a small G-actin binding protein that induces depolymerization of intracellular F-actin pools by sequestering actin monomers. Previously, we demonstrated that overexpression of TB10 in ovarian tumor cells increased the rate of cell death. As an initial step to define molecular mechanism of TB10-dependent apoptotic process in ovarian tumor cells, we searched a human ovary cDNA library for a novel TB10 binding protein using a yeast two-hybrid system. The selected protein was human E-tropomodulin (E-Tmod), another component of the actin binding proteins. Subsequently, two interacting protein components were determined quantitatively. Results showed that the full-length TB10 is required to bind with E-Tmod, and the TB10 binding site on E-Tmod partially overlaps with the actin binding site on E-Tmod. Moreover, introduction of E-Tmod cDNA into a tumor cell line reversed TB10 mediated apoptosis and restored actin architectures. These results may suggest that TB10 regulates apoptotic homeostasis by not only just binding to actin but also competing or blocking the protein complex formation of E-Tmod with actin.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalFEBS Letters
Volume557
Issue number1-3
DOIs
Publication statusPublished - 2004 Jan 16
Externally publishedYes

Fingerprint

Tropomodulin
Thymosin
Actins
Tumors
Cells
Apoptosis
Neoplasms
Microfilament Proteins
Binding Sites
Two-Hybrid System Techniques
Depolymerization
Proteins
Cell death
Tumor Cell Line
Hybrid systems
Gene Library
Yeast
Ovary
Carrier Proteins
Homeostasis

Keywords

  • Actin sequestering protein
  • Apoptosis
  • E-tropomodulin
  • Thymosin β-10
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The interaction between E-tropomodulin and thymosin β-10 rescues tumor cells from thymosin β-10 mediated apoptosis by restoring actin architecture. / Rho, Seung Bae; Chun, Taehoon; Lee, Seung Hoon; Park, Kyoungsook; Lee, Je Ho.

In: FEBS Letters, Vol. 557, No. 1-3, 16.01.2004, p. 57-63.

Research output: Contribution to journalArticle

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