The interaction of phospholipase C-β3 with Shank2 regulates mGluR-mediated calcium signal

Jong Ik Hwang, Soo Kim Hyeon, Ran Lee Jae, Eunjoon Kim, Ho Ryu Sung, Pann Ghill Suh

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)

Abstract

Phospholipase C-β isozymes that are activated by G protein-coupled receptors (GPCR) and heterotrimeric G proteins carry a PSD-95/Dlg/ZO-1 (PDZ) domain binding motif at their C terminus. Through interactions with PDZ domains, this motif may endow the PLC-β isozyme with specific roles in GPCR signaling events that occur in compartmentalized regions of the plasma membrane. In this study, we identified the interaction of PLC-β3 with Shank2, a PDZ domain-containing multimodular scaffold in the postsynaptic density (PSD). The C terminus of PLC-β3, but not other PLC-β isotypes, specifically interacts with the PDZ domain of Shank2. Homer 1b, a Shank-interacting protein that is linked to group I metabotropic glutamate receptors and IP3 receptors, forms a multiple complex with Shank2 and PLC-β3. Importantly, microinjection of a synthetic peptide specifically mimicking the C terminus of PLC-β3 markedly reduces the mGluR-mediated intracellular calcium response. These results demonstrate that Shank2 brings PLC-β3 closer to Homer 1b and constitutes an efficient mGluR-coupled signaling pathway in the PSD region of neuronal synapses.

Original languageEnglish
Pages (from-to)12467-12473
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number13
DOIs
Publication statusPublished - 2005 Apr 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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