The interactions between MicroRNA-200c and BRD7 in endometrial carcinoma

Young Ae Park, Jeong Won Lee, Jung Joo Choi, Hye Kyung Jeon, Youngjae Cho, Chelhun Choi, Tae Joong Kim, Nak Woo Lee, Byoung Gie Kim, Duk Soo Bae

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39 Citations (Scopus)

Abstract

Objective: Increased expression of miR-200c was recently reported in endometrial carcinoma compared with normal tissues. In this study, we evaluated the role of miR-200c in cell growth and drug sensitivity in endometrial carcinoma and investigated the underlying mechanisms. Methods: The expression of miR-200c in human endometrial tissues was detected by quantitative RT-PCR. The transfection with anti-miRNA (anti-miR) or the premature form of miRNA (pre-miR) was performed to regulate the level of expression of miRNA-200c in endometrial carcinoma cells, HEC-1A and Ishikawa. To identify the target genes for miR-200c, we performed mRNA microarray after pre-miR-200c transfection in HEC-1A cells. Results: We found that miR-200c expression was increased in endometrial carcinoma compared with normal endometrial tissues. Anti-miR or pre-miR-200c could regulate cell survival, proliferation, and apoptosis and affect cytotoxicity in endometrial cancer cells. Through mRNA microarray analysis, we found that miR-200c inhibits the expression of BRD7, which was recently reported as a potential tumor suppressor gene. MiR-200c regulated the translocation of β-catenin from the cytoplasm to the nucleus via inhibition of BRD7, resulting in increased expression of its transcriptional target genes, cyclinD1 and c-myc. Conclusion: The interaction between miR-200c and BRD7 might have important roles in controlling growth of endometrial of cancer cells and suggest a novel target pathway for treatment of this cancer.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalGynecologic Oncology
Volume124
Issue number1
DOIs
Publication statusPublished - 2012 Jan

Keywords

  • BRD7
  • Endometrial carcinoma
  • MicroRNA
  • MicroRNA-200c
  • β-catenin

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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  • Cite this

    Park, Y. A., Lee, J. W., Choi, J. J., Jeon, H. K., Cho, Y., Choi, C., Kim, T. J., Lee, N. W., Kim, B. G., & Bae, D. S. (2012). The interactions between MicroRNA-200c and BRD7 in endometrial carcinoma. Gynecologic Oncology, 124(1), 125-133. https://doi.org/10.1016/j.ygyno.2011.09.026