The knockdown of trek-1 in hippocampal neurons attenuate lipopolysaccharide-induced depressive-like behavior in mice

Ajung Kim, Hyun Gug Jung, Yeong Eun Kim, Seung Chan Kim, Jae Yong Park, Seok Geun Lee, Eun Mi Hwang

Research output: Contribution to journalArticle

Abstract

TWIK-related potassium channel-1 (TREK-1) is broadly expressed in the brain and involved in diverse brain diseases, such as seizures, ischemia, and depression. However, the cell type-specific roles of TREK-1 in the brain are largely unknown. Here, we generated a Cre-dependent TREK-1 knockdown (Cd-TREK-1 KD) transgenic mouse containing a gene cassette for Cre-dependent TREK-1 short hairpin ribonucleic acid to regulate the cell type-specific TREK-1 expression. We confirmed the knockdown of TREK-1 by injecting adeno-associated virus (AAV) expressing Cre into the hippocampus of the mice. To study the role of hippocampal neuronal TREK-1 in a lipopolysaccharide (LPS)-induced depression model, we injected AAV-hSyn-BFP (nCTL group) or AAV-hSyn-BFP-Cre (nCre group) virus into the hippocampus of Cd-TREK-1 KD mice. Interestingly, the immobility in the tail suspension test after LPS treatment did not change in the nCre group. Additionally, some neurotrophic factors (BDNF, VEGF, and IGF-1) significantly increased more in the nCre group compared to the nCTL group after LPS treatment, but there was no difference in the expression of their receptors. Therefore, our data suggest that TREK-1 in the hippocampal neurons has antidepressant effects, and that Cd-TREK-1 KD mice are a valuable tool to reveal the cell type-specific roles of TREK-1 in the brain.

Original languageEnglish
Article number5902
JournalInternational journal of molecular sciences
Volume20
Issue number23
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

neurons
Viruses
Neurons
mice
Lipopolysaccharides
viruses
Brain
brain
hippocampus
Dependovirus
cells
ribonucleic acids
seizures
ischemia
RNA
Potassium
Genes
genes
potassium channel protein TREK-1
Hippocampus

Keywords

  • Conditional knockdown
  • Depression
  • Hippocampus
  • Neurotrophic factor
  • TREK-1

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

The knockdown of trek-1 in hippocampal neurons attenuate lipopolysaccharide-induced depressive-like behavior in mice. / Kim, Ajung; Jung, Hyun Gug; Kim, Yeong Eun; Kim, Seung Chan; Park, Jae Yong; Lee, Seok Geun; Hwang, Eun Mi.

In: International journal of molecular sciences, Vol. 20, No. 23, 5902, 01.12.2019.

Research output: Contribution to journalArticle

Kim, Ajung ; Jung, Hyun Gug ; Kim, Yeong Eun ; Kim, Seung Chan ; Park, Jae Yong ; Lee, Seok Geun ; Hwang, Eun Mi. / The knockdown of trek-1 in hippocampal neurons attenuate lipopolysaccharide-induced depressive-like behavior in mice. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 23.
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