The Mach-Zehnder interferometer based on silicon oxides for label free detection of C-reactive protein (CRP)

Jongin Hong, Dae Sung Yoon, Tae Song Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The C-reactive protein (CRP) is a very important inflammation biomarker and a major predictor of cardiovascular disease (CVD) in human. We demonstrated here on a new approach to determine the binding of label free CRP to monoclonal anti-CRP using the Mach-Zehnder interferometer based on silicon oxides with the detection limit of 1 ng/mL or even less at the operating wavelength of 1,550 nm. The building block, single-mode waveguide of total internal reflection, was precisely designed using a finite element method (FEM) and so we suggested the guideline of microfabrication to achieve it. We also firstly introduced the calixcrown self-assembled monolayers (SAMs), which guaranteed the activity of capture proteins and the correct orientation, to immobilize the monoclonal anti-CRP on PECVD silicon oxides into the biosensors based on the interaction of the evanescent field of the guided light with the surrounding environment.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalBiochip Journal
Volume3
Issue number1
Publication statusPublished - 2009 Jan 1
Externally publishedYes

Fingerprint

Mach-Zehnder interferometers
Silicon oxides
Silicon
C-Reactive Protein
Oxides
Labels
Proteins
Microtechnology
Evanescent fields
Microfabrication
Biosensing Techniques
Self assembled monolayers
Biomarkers
Plasma enhanced chemical vapor deposition
Biosensors
Limit of Detection
Waveguides
Cardiovascular Diseases
Guidelines
Inflammation

Keywords

  • Biosensor
  • C-reactive protein
  • Finite element method
  • Mach-zehnder interferometer
  • Silicon oxide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

The Mach-Zehnder interferometer based on silicon oxides for label free detection of C-reactive protein (CRP). / Hong, Jongin; Yoon, Dae Sung; Kim, Tae Song.

In: Biochip Journal, Vol. 3, No. 1, 01.01.2009, p. 1-11.

Research output: Contribution to journalArticle

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