The mechanisms of action of E.coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites

Joon Kim, Stuart Linn

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Treatment of DNA containing AP sites with either T4 UV endonuclease or with E. coli endonuclease III followed by a human class II AP endonuclease releases a putative β-elimination product. This result suggests that both the T4 endonuclease and E. coli endonuclease III class I AP endonucleases catalyze pbosphodiester bond cleavage via a lyase- rathar than a hydrolase mechanism. Indeed, we have not detected a class I AP endonuclease which hydrolytically catalyzes phosphodiester bond cleavage. Whereas these enzymes use a lyase-like rather than a hydrolytic mechanism, they nonetheless catalyze phosphodiester bond cleavage. We suggest that the term endonuclease can be properly applied to them.

Original languageEnglish
Pages (from-to)1135-1141
Number of pages7
JournalNucleic Acids Research
Volume16
Issue number3
DOIs
Publication statusPublished - 1988 Feb 11
Externally publishedYes

Fingerprint

Deoxyribonuclease (Pyrimidine Dimer)
DNA-(Apurinic or Apyrimidinic Site) Lyase
Escherichia coli
Escherichia Coli
Lyases
Endonucleases
Hydrolases
Elimination
Enzymes
DNA
Term
E coli NTH protein
UV endonuclease
Class

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The mechanisms of action of E.coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites. / Kim, Joon; Linn, Stuart.

In: Nucleic Acids Research, Vol. 16, No. 3, 11.02.1988, p. 1135-1141.

Research output: Contribution to journalArticle

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