The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors

Michiko Echizenya, Shunya Kondo, Rei Takahashi, Jun Seo Oh, Satoshi Kawashima, Hitoshi Kitayama, Chiaki Takahashi, Makoto Noda

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The membrane-anchored MMP-regulator RECK is down regulated in many solid tumors; the extent of RECK down regulation correlates with poor prognosis. Forced expression of RECK in tumor cells results in suppression of angiogenesis, invasion, and metastasis. Studies on the roles and the mechanisms of regulation of the RECK gene during normal development may therefore yield important insights into how the malignant behaviors of tumor cells arise and how they can be controlled. Our previous studies indicate that mice lacking RECK die around E10.5 with reduced tissue integrity. In the present study, we have found that in later stage wild-type embryos, RECK is abundantly expressed in skeletal muscles, especially in the areas where the myoblast differentiation factor MRF4 is expressed. Consistent with this finding, the RECK-promoter is activated by MRF4 in cultured cells. In contrast, a myoblast determination factor MyoD suppresses the RECK-promoter. Myoblastic cells lacking RECK expression give rise to myotubes at higher efficiency than the cells expressing RECK, indicating that RECK suppresses myotube formation. These findings suggest that MyoD down regulates RECK to facilitate myotube formation, whereas MRF4 up regulates RECK to promote other aspects of myogenesis that require extracellular matrix integrity.

Original languageEnglish
Pages (from-to)5850-5857
Number of pages8
JournalOncogene
Volume24
Issue number38
DOIs
Publication statusPublished - 2005 Sep 1

Fingerprint

Myogenic Regulatory Factors
Matrix Metalloproteinases
Skeletal Muscle Fibers
Membranes
Myoblasts
MyoD Protein
Down-Regulation
Neoplasms
Muscle Development
Extracellular Matrix
Cultured Cells
Skeletal Muscle
Up-Regulation
Embryonic Structures
Neoplasm Metastasis
Genes
myogenic factor 6

Keywords

  • MRF4
  • MyoD
  • Myogenesis
  • RECK

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Echizenya, M., Kondo, S., Takahashi, R., Oh, J. S., Kawashima, S., Kitayama, H., ... Noda, M. (2005). The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors. Oncogene, 24(38), 5850-5857. https://doi.org/10.1038/sj.onc.1208733

The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors. / Echizenya, Michiko; Kondo, Shunya; Takahashi, Rei; Oh, Jun Seo; Kawashima, Satoshi; Kitayama, Hitoshi; Takahashi, Chiaki; Noda, Makoto.

In: Oncogene, Vol. 24, No. 38, 01.09.2005, p. 5850-5857.

Research output: Contribution to journalArticle

Echizenya, M, Kondo, S, Takahashi, R, Oh, JS, Kawashima, S, Kitayama, H, Takahashi, C & Noda, M 2005, 'The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors', Oncogene, vol. 24, no. 38, pp. 5850-5857. https://doi.org/10.1038/sj.onc.1208733
Echizenya M, Kondo S, Takahashi R, Oh JS, Kawashima S, Kitayama H et al. The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors. Oncogene. 2005 Sep 1;24(38):5850-5857. https://doi.org/10.1038/sj.onc.1208733
Echizenya, Michiko ; Kondo, Shunya ; Takahashi, Rei ; Oh, Jun Seo ; Kawashima, Satoshi ; Kitayama, Hitoshi ; Takahashi, Chiaki ; Noda, Makoto. / The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors. In: Oncogene. 2005 ; Vol. 24, No. 38. pp. 5850-5857.
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