The modulation of motility of pyloric antral smooth muscles of rat by melatonin

Sang Hoon Han, Woon da Lee, Soo Hyun Cho, Junesun Kim

Research output: Contribution to journalArticle

Abstract

Recently some researches have established that the melatonin, secreted by pineal gland, may evoke the changes of contractile responses on smooth muscles. We examined the effects of melatonin on the motility of rat pyloric antrum and which mechanism might be involved in the effects. Pyloric antral strips from the stomach of 20 Sprague-Dawley rats were prepared for isometric tension recording in organ bath. The strips were precontracted by acetylcholine and high-KCl solutions. In precontracted conditions the tensions were increased by accumulative application of melatonin (10-8-10-4 M) dose-dependently, even in resting states. And the effects were almost disappeared when the concentrations of ACh were over than 10 μM. The effects of melatonin were inhibited by pretreatment of 10 mM TEA and/or 10 μM 4-AP and rarely affected by pretreatment of 1 mM TEA, 10 μM glibenclamide and 10 μM verapamil respectively. From these results it is concluded that the contractile responses of smooth muscles of rat pyloric antrum were enhanced by melatonin application and the mechanism might be concerned with the inhibition of some voltage-dependent potassium channels.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalBiomolecules and Therapeutics
Volume18
Issue number2
DOIs
Publication statusPublished - 2010 Jun 9

Fingerprint

Melatonin
Smooth Muscle
Muscle
Rats
Modulation
Pyloric Antrum
Pineal Gland
Glyburide
Potassium Channels
Verapamil
Baths
Acetylcholine
Sprague Dawley Rats
Stomach
Antral
Electric potential
Research

Keywords

  • Melatonin
  • Potassium channel
  • Pyloric antrum
  • Smooth muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

Cite this

The modulation of motility of pyloric antral smooth muscles of rat by melatonin. / Han, Sang Hoon; da Lee, Woon; Cho, Soo Hyun; Kim, Junesun.

In: Biomolecules and Therapeutics, Vol. 18, No. 2, 09.06.2010, p. 166-170.

Research output: Contribution to journalArticle

Han, Sang Hoon ; da Lee, Woon ; Cho, Soo Hyun ; Kim, Junesun. / The modulation of motility of pyloric antral smooth muscles of rat by melatonin. In: Biomolecules and Therapeutics. 2010 ; Vol. 18, No. 2. pp. 166-170.
@article{0414c37434bb4359ac1217b66b88bdb1,
title = "The modulation of motility of pyloric antral smooth muscles of rat by melatonin",
abstract = "Recently some researches have established that the melatonin, secreted by pineal gland, may evoke the changes of contractile responses on smooth muscles. We examined the effects of melatonin on the motility of rat pyloric antrum and which mechanism might be involved in the effects. Pyloric antral strips from the stomach of 20 Sprague-Dawley rats were prepared for isometric tension recording in organ bath. The strips were precontracted by acetylcholine and high-KCl solutions. In precontracted conditions the tensions were increased by accumulative application of melatonin (10-8-10-4 M) dose-dependently, even in resting states. And the effects were almost disappeared when the concentrations of ACh were over than 10 μM. The effects of melatonin were inhibited by pretreatment of 10 mM TEA and/or 10 μM 4-AP and rarely affected by pretreatment of 1 mM TEA, 10 μM glibenclamide and 10 μM verapamil respectively. From these results it is concluded that the contractile responses of smooth muscles of rat pyloric antrum were enhanced by melatonin application and the mechanism might be concerned with the inhibition of some voltage-dependent potassium channels.",
keywords = "Melatonin, Potassium channel, Pyloric antrum, Smooth muscle",
author = "Han, {Sang Hoon} and {da Lee}, Woon and Cho, {Soo Hyun} and Junesun Kim",
year = "2010",
month = "6",
day = "9",
doi = "10.4062/biomolther.2010.18.2.166",
language = "English",
volume = "18",
pages = "166--170",
journal = "Biomolecules and Therapeutics",
issn = "1976-9148",
publisher = "Korean Society of Applied Pharmacology",
number = "2",

}

TY - JOUR

T1 - The modulation of motility of pyloric antral smooth muscles of rat by melatonin

AU - Han, Sang Hoon

AU - da Lee, Woon

AU - Cho, Soo Hyun

AU - Kim, Junesun

PY - 2010/6/9

Y1 - 2010/6/9

N2 - Recently some researches have established that the melatonin, secreted by pineal gland, may evoke the changes of contractile responses on smooth muscles. We examined the effects of melatonin on the motility of rat pyloric antrum and which mechanism might be involved in the effects. Pyloric antral strips from the stomach of 20 Sprague-Dawley rats were prepared for isometric tension recording in organ bath. The strips were precontracted by acetylcholine and high-KCl solutions. In precontracted conditions the tensions were increased by accumulative application of melatonin (10-8-10-4 M) dose-dependently, even in resting states. And the effects were almost disappeared when the concentrations of ACh were over than 10 μM. The effects of melatonin were inhibited by pretreatment of 10 mM TEA and/or 10 μM 4-AP and rarely affected by pretreatment of 1 mM TEA, 10 μM glibenclamide and 10 μM verapamil respectively. From these results it is concluded that the contractile responses of smooth muscles of rat pyloric antrum were enhanced by melatonin application and the mechanism might be concerned with the inhibition of some voltage-dependent potassium channels.

AB - Recently some researches have established that the melatonin, secreted by pineal gland, may evoke the changes of contractile responses on smooth muscles. We examined the effects of melatonin on the motility of rat pyloric antrum and which mechanism might be involved in the effects. Pyloric antral strips from the stomach of 20 Sprague-Dawley rats were prepared for isometric tension recording in organ bath. The strips were precontracted by acetylcholine and high-KCl solutions. In precontracted conditions the tensions were increased by accumulative application of melatonin (10-8-10-4 M) dose-dependently, even in resting states. And the effects were almost disappeared when the concentrations of ACh were over than 10 μM. The effects of melatonin were inhibited by pretreatment of 10 mM TEA and/or 10 μM 4-AP and rarely affected by pretreatment of 1 mM TEA, 10 μM glibenclamide and 10 μM verapamil respectively. From these results it is concluded that the contractile responses of smooth muscles of rat pyloric antrum were enhanced by melatonin application and the mechanism might be concerned with the inhibition of some voltage-dependent potassium channels.

KW - Melatonin

KW - Potassium channel

KW - Pyloric antrum

KW - Smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=77953112422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953112422&partnerID=8YFLogxK

U2 - 10.4062/biomolther.2010.18.2.166

DO - 10.4062/biomolther.2010.18.2.166

M3 - Article

AN - SCOPUS:77953112422

VL - 18

SP - 166

EP - 170

JO - Biomolecules and Therapeutics

JF - Biomolecules and Therapeutics

SN - 1976-9148

IS - 2

ER -