The mRNP remodeling mediated by UPF1 promotes rapid degradation of replication-dependent histone mRNA

Junho Choe, Sang Ho Ahn, Yoon Ki Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Histone biogenesis is tightly controlled at multiple steps to maintain the balance between the amounts of DNA and histone protein during the cell cycle. In particular, translation and degradation of replication-dependent histone mRNAs are coordinately regulated. However, the underlying molecular mechanisms remain elusive. Here, we investigate remodeling of stem-loop binding protein (SLBP)-containing histone mRNPs occurring during the switch from the actively translating mode to the degradation mode. The interaction between a CBP80/20-dependent translation initiation factor (CTIF) and SLBP, which is important for efficient histone mRNA translation, is disrupted upon the inhibition of DNA replication or at the end of S phase. This disruption is mediated by competition between CTIF and UPF1 for SLBP binding. Further characterizations reveal hyperphosphorylation of UPF1 by activated ATR and DNA-dependent protein kinase upon the inhibition of DNA replication interacts with SLBP more strongly, promoting the release of CTIF and eIF3 from SLBP-containing histone mRNP. In addition, hyperphosphorylated UPF1 recruits PNRC2 and SMG5, triggering decapping followed by 5′-to-3′ degradation of histone mRNAs. The collective observations suggest that both inhibition of translation and recruitment of mRNA degradation machinery during histone mRNA degradation are tightly coupled and coordinately regulated by UPF1 phosphorylation.

Original languageEnglish
Pages (from-to)9334-9349
Number of pages16
JournalNucleic Acids Research
Volume42
Issue number14
DOIs
Publication statusPublished - 2014 Jan 1

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Histones
Degradation
Messenger RNA
Carrier Proteins
Peptide Initiation Factors
RNA Stability
DNA Replication
DNA
DNA-Activated Protein Kinase
Cell Cycle Proteins
Phosphorylation
messenger ribonucleoprotein
Protein Biosynthesis
S Phase
Protein Binding
Machinery
Cells
Switches
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

The mRNP remodeling mediated by UPF1 promotes rapid degradation of replication-dependent histone mRNA. / Choe, Junho; Ahn, Sang Ho; Kim, Yoon Ki.

In: Nucleic Acids Research, Vol. 42, No. 14, 01.01.2014, p. 9334-9349.

Research output: Contribution to journalArticle

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