The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences

Minyoung Hwang, Marat Gorivodsky, Minjung Kim, Heiner Westphal, Dongho Geum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

LIM-domain binding protein 1 (Ldb1) is a multiadaptor protein that mediates the action of transcription factors, including LIM-homeodomain proteins. To elucidate the functional role of Ldb1 in the neuronal differentiation of embryonic stem (ES) cells, we have generated Ldb1-null mutant (Ldb1-/-) ES cells and examined neuronal differentiation potentials in vitro using two different neuronal differentiation protocols. When subjected to a five-stage protocol that recapitulates in vivo conditions of neuronal differentiation, wild-type ES cells differentiated into a wide spectrum of neuronal cell types. However, Ldb1-/- ES cells did not differentiate into neuronal cells; instead, they differentiated into sarcomeric α-actinin-positive muscle cells. In contrast, when an adherent monolayer culture procedure (which is based on the default mechanism of neural induction and eliminates environmental influences) was applied, both wild-type and Ldb1-/- ES cells differentiated into MAP2-positive mature neurons. Comparison of the results obtained when two different neuronal differentiation protocols were used suggests that Ldb1-/- ES cells have an innate potential to differentiate into neuronal cells, but this potential can be inhibited by environmental influences.

Original languageEnglish
Pages (from-to)1490-1495
Number of pages6
JournalStem Cells
Volume26
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

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Keywords

  • Adherent monolayer culture method
  • Embryoid body
  • Embryonic stem cells
  • Five-stage method
  • Ldb1
  • Neuronal differentiation

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine
  • Medicine(all)

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