The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans

M. D. Nelson, N. F. Trojanowski, J. B. George-Raizen, C. J. Smith, C. C. Yu, Christopher Fang-Yen, D. M. Raizen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Neuropeptides have central roles in the regulation of homoeostatic behaviours such as sleep and feeding. Caenorhabditis elegans displays sleep-like quiescence of locomotion and feeding during a larval transition stage called lethargus and feeds during active larval and adult stages. Here we show that the neuropeptide NLP-22 is a regulator of Caenorhabditis elegans sleep-like quiescence observed during lethargus. nlp-22 shows cyclical mRNA expression in synchrony with lethargus; it is regulated by LIN-42, an orthologue of the core circadian protein PERIOD; and it is expressed solely in the two RIA interneurons. nlp-22 and the RIA interneurons are required for normal lethargus quiescence, and forced expression of nlp-22 during active stages causes anachronistic locomotion and feeding quiescence. Optogenetic stimulation of the RIA interneurons has a movement-promoting effect, demonstrating functional complexity in a single-neuron type. Our work defines a quiescence-regulating role for NLP-22 and expands our knowledge of the neural circuitry controlling Caenorhabditis elegans behavioural quiescence.

Original languageEnglish
Article number2846
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013 Dec 4

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sleep
Caenorhabditis elegans
Interneurons
Neuropeptides
Sleep
locomotion
Locomotion
Optogenetics
regulators
neurons
stimulation
Neurons
proteins
Messenger RNA
causes
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Nelson, M. D., Trojanowski, N. F., George-Raizen, J. B., Smith, C. J., Yu, C. C., Fang-Yen, C., & Raizen, D. M. (2013). The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. Nature Communications, 4, [2846]. https://doi.org/10.1038/ncomms3846

The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. / Nelson, M. D.; Trojanowski, N. F.; George-Raizen, J. B.; Smith, C. J.; Yu, C. C.; Fang-Yen, Christopher; Raizen, D. M.

In: Nature Communications, Vol. 4, 2846, 04.12.2013.

Research output: Contribution to journalArticle

Nelson, MD, Trojanowski, NF, George-Raizen, JB, Smith, CJ, Yu, CC, Fang-Yen, C & Raizen, DM 2013, 'The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans', Nature Communications, vol. 4, 2846. https://doi.org/10.1038/ncomms3846
Nelson MD, Trojanowski NF, George-Raizen JB, Smith CJ, Yu CC, Fang-Yen C et al. The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. Nature Communications. 2013 Dec 4;4. 2846. https://doi.org/10.1038/ncomms3846
Nelson, M. D. ; Trojanowski, N. F. ; George-Raizen, J. B. ; Smith, C. J. ; Yu, C. C. ; Fang-Yen, Christopher ; Raizen, D. M. / The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. In: Nature Communications. 2013 ; Vol. 4.
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