The ORF49 protein of murine gammaherpesvirus 68 cooperates with RTA in regulating virus replication

Sangmi Lee, Hye Jeong Cho, Jung Jin Park, Yong Sun Kim, Seungmin Hwang, Ren Sun, Jung Song Moon

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16 Citations (Scopus)


Our functional mapping study of murine gammaherpesvirus 68 (MHV-68, or γHV-68) revealed that a mutant harboring a transposon at the ORF49 locus (ORF49null) evidenced a highly attenuated in vitro growth. ORF49 resides adjacent to and in an opposite direction from RTA, the primary switch of the gammaherpesvirus life cycle. A FLAG-tagged ORF49 protein was able to transcomplement ORF49null, and a revertant of ORF49null restored its attenuated growth to a level comparable to that of the wild type. The FLAG-tagged ORF49 protein promoted the ability of RTA to activate downstream target promoters and enhanced virus replication from the ORF50null virus in the presence of RTA. Furthermore, ORF49 enhanced wild-type virus replication by increasing the RTA transcript levels. Our data indicate that ORF49 may perform an important function in MHV-68 replication in cooperation with RTA.

Original languageEnglish
Pages (from-to)9870-9877
Number of pages8
JournalJournal of virology
Issue number18
Publication statusPublished - 2007 Sep 1


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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