The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus

Jin Il Kim, Min Woong Hwang, Ilseob Lee, Sehee Park, Sangmoo Lee, Joon Yong Bae, Jun Heo, Donghwan Kim, Seok Il Jang, Mee Sook Park, Hyung Joo Kwon, Jin Won Song, Man Seong Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

By nature of their segmented RNA genome, influenza A viruses (IAVs) have the potential to generate variants through a reassortment process. The influenza nonstructural (NS) gene is critical for a virus to counteract the antiviral responses of the host. Therefore, a newly acquired NS segment potentially determines the replication efficiency of the reassortant virus in a range of different hosts. In addition, the C-terminal PDZ-binding motif (PBM) has been suggested as a pathogenic determinant of IAVs. To gauge the pandemic potential from human and avian IAV reassortment, we assessed the replication properties of NS-reassorted viruses in cultured cells and in the lungs of mice and determined their transmissibility in guinea pigs. Compared with the recombinant A/Korea/01/2009 virus (rK09; 2009 pandemic H1N1 strain), the rK09/VN:NS virus, in which the NS gene was adopted from the A/Vietnam/1203/2004 virus (a human isolate of the highly pathogenic avian influenza H5N1 virus strains), exhibited attenuated virulence and reduced transmissibility. However, the rK09/VN:NS-PBM virus, harboring the PBM in the C-terminus of the NS1 protein, recovered the attenuated virulence of the rK09/VN:NS virus. In a guinea pig model, the rK09/VN:NS-PBM virus showed even greater transmission efficiency than the rK/09 virus. These results suggest that the PBM in the NS1 protein may determine viral persistence in the human and avian IAV interface.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalBiochemical and biophysical research communications
Volume449
Issue number1
DOIs
Publication statusPublished - 2014 Jun 20

Keywords

  • Influenza A virus
  • Interferon
  • NS1 protein
  • PDZ-binding motif
  • Transmission

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus'. Together they form a unique fingerprint.

Cite this