The position of the target site for engineered nucleases improves the aberrant mRNA clearance in in vivo genome editing

Jae Hoon Lee, Sungsook Yu, Tae Wook Nam, Jae il Roh, Young Jin, Jeong Pil Han, Ji Young Cha, Yoon Ki Kim, Su Cheong Yeom, Ki Taek Nam, Han Woong Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Engineered nucleases are widely used for creating frameshift or nonsense mutations in the target genes to eliminate gene functions. The resulting mRNAs carrying premature termination codons can be eliminated by nonsense-mediated mRNA decay. However, it is unclear how effective this process would be in vivo. Here, we found that the nonsense-mediated decay was unable to remove the mutant mRNAs in twelve out of sixteen homozygous mutant mice with frameshift mutations generated using engineered nucleases, which is far beyond what we expected. The frameshift mutant proteins translated by a single nucleotide deletion within the coding region were also detected in the p53 mutant mice. Furthermore, we showed that targeting the exons present downstream of the exons with a start codon or distant from ATG is relatively effective for eliminating mutant mRNAs in vivo, whereas the exons with a start codon are targeted to express the mutant mRNAs. Of the sixteen mutant mice generated, only four mutant mice targeting the downstream exons exhibited over 80% clearance of mutant mRNAs. Since the abnormal products, either mutant RNAs or mutant proteins, expressed by the target alleles might obscure the outcome of genome editing, these findings will provide insights in the improved performance of engineered nucleases when they are applied in vivo.

Original languageEnglish
Article number4173
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The position of the target site for engineered nucleases improves the aberrant mRNA clearance in in vivo genome editing'. Together they form a unique fingerprint.

Cite this